Detailed information NTG0428048

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: G8765_RS05295
• Genome accession: NZ_CP049828
• Coordinates: 1189801..1190940 (-)
• Length: 379 a.a.
• NCBI ID: WP_176374706.1
• Uniprot ID: -
• Organism: Janthinobacterium lividum strain EIF2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1184801..1195940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G8765_RS05280 (G8765_05285)	glcF	1185785..1187023 (+)	1239	WP_176381849.1	glycolate oxidase subunit GlcF	-
G8765_RS05285 (G8765_05290)	-	1187016..1189127 (-)	2112	WP_176381851.1	EAL domain-containing protein	-
G8765_RS05290 (G8765_05295)	-	1189314..1189799 (-)	486	WP_176381853.1	glutathione peroxidase	-
G8765_RS05295 (G8765_05300)	pilU	1189801..1190940 (-)	1140	WP_176374706.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
G8765_RS05300 (G8765_05305)	pilT	1190944..1191987 (-)	1044	WP_035824693.1	type IV pilus twitching motility protein PilT	Machinery gene
G8765_RS05305 (G8765_05310)	-	1192058..1192759 (+)	702	WP_070278361.1	YggS family pyridoxal phosphate-dependent enzyme	-
G8765_RS05310 (G8765_05315)	proC	1192782..1193612 (+)	831	WP_092612864.1	pyrroline-5-carboxylate reductase	-
G8765_RS05315 (G8765_05320)	-	1193613..1194329 (-)	717	WP_092612867.1	anti-sigma factor	-
G8765_RS05320 (G8765_05325)	-	1194326..1194883 (-)	558	WP_092613087.1	sigma-70 family RNA polymerase sigma factor	-
G8765_RS05325 (G8765_05330)	-	1195100..1195657 (-)	558	WP_034784851.1	DUF3455 domain-containing protein	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 41079.19 Da
• Isoelectric Point: 6.9059

>NTDB_id=428048 G8765_RS05295 WP_176374706.1 1189801..1190940(-) (pilU) [Janthinobacterium lividum strain EIF2]
MQTTHEHYGAMHALLAQMRARSGSDLFITAGFPPAIKLDGKLTPLAGGALDAQQSAGYVRAVMNERQAAEFASSREANFA
ISPDGLGRFRVSAFVQMGQAGMVLRLINTAIPTLDGLGLPPILQDIVMSKRGLVIMVGATGCGKSTTLAAMVGHRNAHSH
GHIITIEDPVEFIHPHGNCIVTQREVGVDTDDWATALKNTLRQAPDVIQIGEIRDRDTMDHAIAFAETGHLCLATLHANN
ANQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPSKEGGGRKAALEILLNSPLMSDLIFKGQVHEIKELMKKSREH
GMQTFDQALFDLHEGGAISYEDALRNADSVNDLRLTIKLKGATAPEPPPQTGATKLGLL

Nucleotide

• Length: 1140 bp

>NTDB_id=428048 G8765_RS05295 WP_176374706.1 1189801..1190940(-) (pilU) [Janthinobacterium lividum strain EIF2]
ATGCAGACGACGCACGAACACTATGGCGCCATGCACGCGCTGCTGGCGCAGATGCGCGCACGGAGCGGCTCGGACCTGTT
CATCACGGCCGGCTTTCCGCCCGCCATCAAGCTCGACGGCAAGCTGACGCCGCTGGCTGGCGGCGCGCTCGACGCGCAGC
AATCGGCCGGCTACGTGCGCGCCGTCATGAACGAACGCCAGGCGGCCGAGTTCGCGTCCAGCCGCGAAGCCAATTTCGCC
ATCAGCCCGGACGGTCTCGGACGTTTCCGCGTATCCGCCTTCGTGCAGATGGGCCAGGCAGGCATGGTCTTGCGCTTGAT
CAATACCGCCATCCCCACCCTCGATGGACTGGGTTTGCCCCCCATCCTGCAAGACATCGTGATGAGCAAGCGGGGCCTCG
TCATCATGGTGGGCGCCACGGGCTGCGGCAAGTCGACCACGCTGGCGGCCATGGTGGGCCACCGCAACGCGCACAGCCAC
GGCCACATCATCACCATCGAAGACCCCGTGGAATTCATCCATCCGCACGGCAACTGCATCGTCACCCAGCGCGAAGTGGG
CGTCGATACGGATGACTGGGCCACGGCCCTGAAAAACACCCTGCGCCAGGCGCCCGACGTGATACAGATCGGAGAAATAC
GCGACCGCGACACCATGGACCACGCCATCGCGTTTGCCGAAACGGGCCATCTGTGCCTGGCGACCTTGCACGCGAACAAC
GCCAACCAGGCGCTGGACCGCATCATCAATTTCTTCCCCGAAGAGCGGCGCCAGCAATTGCTGATGGACCTGTCGCTCAA
TTTGAAGGGCATGATTTCGCAGCGCTTGATTCCCAGCAAGGAAGGCGGCGGGCGCAAGGCCGCGCTGGAAATCCTGCTCA
ATTCGCCGCTGATGAGCGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAACTGATGAAAAAATCGCGCGAGCAT
GGCATGCAAACCTTCGACCAGGCCCTGTTCGACCTGCACGAAGGGGGCGCCATCAGCTATGAAGACGCGCTGCGCAATGC
GGACTCCGTCAACGACCTGCGCCTGACGATCAAACTCAAGGGCGCAACGGCGCCGGAACCGCCCCCGCAAACGGGCGCCA
CCAAACTGGGACTCCTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (88-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.173	93.14	0.588
pilU	Acinetobacter baylyi ADP1	59.942	91.557	0.549
pilU	Vibrio cholerae strain A1552	56.851	90.501	0.515
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.119	86.28	0.372
pilT	Acinetobacter baylyi ADP1	41.515	87.071	0.361