Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GTQ41_RS02140 Genome accession   NZ_CP049737
Coordinates   475447..476511 (+) Length   354 a.a.
NCBI ID   WP_047290890.1    Uniprot ID   A0A2A2PKF2
Organism   Pseudomonas sp. AN-B15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470447..481511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ41_RS02130 (GTQ41_02130) - 471279..472547 (+) 1269 WP_041074924.1 malic enzyme-like NAD(P)-binding protein -
  GTQ41_RS02135 (GTQ41_02135) - 472783..475230 (-) 2448 WP_047290889.1 penicillin-binding protein 1A -
  GTQ41_RS02140 (GTQ41_02140) pilM 475447..476511 (+) 1065 WP_047290890.1 pilus assembly protein PilM Machinery gene
  GTQ41_RS02145 (GTQ41_02145) - 476511..477077 (+) 567 WP_047290891.1 PilN domain-containing protein -
  GTQ41_RS02150 (GTQ41_02150) pilO 477074..477697 (+) 624 WP_047290892.1 type 4a pilus biogenesis protein PilO -
  GTQ41_RS02155 (GTQ41_02155) - 477694..478221 (+) 528 WP_047290893.1 pilus assembly protein PilP -
  GTQ41_RS02160 (GTQ41_02160) pilQ 478235..480307 (+) 2073 WP_047290894.1 type IV pilus secretin PilQ Machinery gene
  GTQ41_RS02165 (GTQ41_02165) aroK 480312..480830 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38119.61 Da        Isoelectric Point: 4.8138

>NTDB_id=427408 GTQ41_RS02140 WP_047290890.1 475447..476511(+) (pilM) [Pseudomonas sp. AN-B15]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGNALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYHSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMSLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=427408 GTQ41_RS02140 WP_047290890.1 475447..476511(+) (pilM) [Pseudomonas sp. AN-B15]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTTTTGGAACT
GAGCCGTCAGGGTGACCGCTACCGCGTCGAGGCCTACGCGGTTGAGCCGCTGCCGCCCAACGCCGTGGTTGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCAATGCCTTGTCCCGCGTGCTGGTCAAGGCGCGGACCGGCCTCAAAAGCGTGGCGGTG
GCGGTGGCCGGTTCAGCGGTGATTACCAAGATCATCGAGATGGATGCCGGGCTGACCGATGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCGCTGGACGAGGTCGCCATCGATTTCGAGGTCCAGGGTGTGTCGCCGC
GCAACCCGGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAGAATGTCGAAGTGCGCGAGGCAGCGCTGGCGCTG
GCCGGGCTGACTGCGCGCGTGGTCGACGTCGAGGCCTACGCGCTGGAACGCTCGTTCGGCCTGCTCGCCACTCAATTGGC
CGCCTCGCAGGAGCGCCTGACTGTCGCCGTGGTGGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGCCGGCAACTGACCGAGGAAATCCAGCGCCGCTACGGTCTGACCGTC
GAGCAGGCCGGACTGGCGAAAAAGCAGGGCGGTCTGCCGGATGACTATGTCAGCGAGGTGTTGCAGCCATTCCGTGACGC
CCTGGTGCAGCAGGTCTCGCGCTCATTGCAGTTCTTTTTCGCCTCCGGGCAGTACCATTCGGTCGATCATATTCTGCTGG
CCGGCGGCACAGCCTCGGTACCGGGGCTGGATCGCCTGATCGAGCAGCGTCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGTCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2PKF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489