Detailed information NTG0042600

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GYO_RS35070
• Genome accession: NC_016047
• Coordinates: 2621529..2622146 (-)
• Length: 205 a.a.
• NCBI ID: WP_014114473.1
• Uniprot ID: G4NXB9
• Organism: Bacillus spizizenii TU-B-10
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2616529..2627146

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GYO_RS35045 (GYO_2823)	rpsT	2616830..2617096 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
GYO_RS35050 (GYO_2824)	holA	2617111..2618154 (-)	1044	WP_014114470.1	DNA polymerase III subunit delta	-
GYO_RS43765 (GYO_2825)	-	2618194..2618343 (-)	150	WP_003229985.1	hypothetical protein	-
GYO_RS35055 (GYO_2826)	-	2618384..2618518 (+)	135	WP_003229983.1	YqzM family protein	-
GYO_RS35060 (GYO_2827)	comEC	2618559..2620889 (-)	2331	WP_041521009.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GYO_RS35065 (GYO_2828)	-	2620893..2621462 (-)	570	WP_014114472.1	ComE operon protein 2	-
GYO_RS35070 (GYO_2829)	comEA	2621529..2622146 (-)	618	WP_014114473.1	helix-hairpin-helix domain-containing protein	Machinery gene
GYO_RS35075 (GYO_2830)	comER	2622230..2623051 (+)	822	WP_014114474.1	late competence protein ComER	-
GYO_RS35080 (GYO_2831)	-	2623117..2623860 (-)	744	WP_014114475.1	class I SAM-dependent methyltransferase	-
GYO_RS35085 (GYO_2832)	rsfS	2623857..2624213 (-)	357	WP_003237112.1	ribosome silencing factor	-
GYO_RS35090 (GYO_2833)	yqeK	2624231..2624791 (-)	561	WP_014114476.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GYO_RS35095 (GYO_2834)	-	2624781..2625350 (-)	570	WP_014114477.1	nicotinate-nucleotide adenylyltransferase	-
GYO_RS35100 (GYO_2835)	yhbY	2625362..2625652 (-)	291	WP_014114478.1	ribosome assembly RNA-binding protein YhbY	-
GYO_RS35105 (GYO_2836)	aroE	2625646..2626488 (-)	843	WP_014114479.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22055.93 Da
• Isoelectric Point: 4.9739

>NTDB_id=42600 GYO_RS35070 WP_014114473.1 2621529..2622146(-) (comEA) [Bacillus spizizenii TU-B-10]
MNWLNQHKKAIILAASAAVFIAVIFFLASGKNKEPMKQTVSTETENTEVKQEAFKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAELLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEQLQGISGV
GPSKAEAIIAYREENGRYQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=42600 GYO_RS35070 WP_014114473.1 2621529..2622146(-) (comEA) [Bacillus spizizenii TU-B-10]
ATGAATTGGTTGAACCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGGTTATTTTCTTCCT
GGCCAGTGGGAAAAACAAAGAGCCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAT
TCAAAGACGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCATCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCTCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGA
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGTGATGGAGGGAAGGGGGCGCTGGTAAATATCAATACTGCAACCTTGGAGCAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTACCAGACAATTGAAGATATCACGAAGGT
TTCAGGAATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

3D structure

• AlphaFold DB: G4NXB9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.756	100	0.898
comEA	Staphylococcus aureus MW2	36.406	100	0.385
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.321	100	0.376
comEA	Staphylococcus aureus N315	34.101	100	0.361