Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XACM_RS15455 Genome accession   NC_016010
Coordinates   3632524..3634260 (-) Length   578 a.a.
NCBI ID   WP_014090854.1    Uniprot ID   -
Organism   Xanthomonas euvesicatoria pv. citrumelo F1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3627524..3639260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XACM_RS15435 (XACM_3134) sucC 3627560..3628729 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  XACM_RS15440 (XACM_3135) - 3628961..3630574 (+) 1614 WP_014090853.1 HAMP domain-containing sensor histidine kinase -
  XACM_RS15450 (XACM_3136) pilR 3630841..3632295 (+) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  XACM_RS15455 (XACM_3137) pilB 3632524..3634260 (-) 1737 WP_014090854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XACM_RS22200 - 3634303..3634713 (-) 411 WP_029820067.1 pilin -
  XACM_RS15465 (XACM_3138) pilA 3634898..3635308 (-) 411 WP_014090855.1 pilin Machinery gene
  XACM_RS15470 (XACM_3139) pilC 3635642..3636898 (+) 1257 WP_014090856.1 type II secretion system F family protein Machinery gene
  XACM_RS15475 (XACM_3140) - 3636905..3637768 (+) 864 WP_008576868.1 A24 family peptidase -
  XACM_RS15480 (XACM_3141) coaE 3637782..3638387 (+) 606 WP_014090857.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62535.58 Da        Isoelectric Point: 5.6006

>NTDB_id=42394 XACM_RS15455 WP_014090854.1 3632524..3634260(-) (pilB) [Xanthomonas euvesicatoria pv. citrumelo F1]
MNAVSSANLVGITGIARRLVQDGAIEEPAARAAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=42394 XACM_RS15455 WP_014090854.1 3632524..3634260(-) (pilB) [Xanthomonas euvesicatoria pv. citrumelo F1]
ATGAATGCTGTTTCGTCCGCTAATTTGGTTGGCATCACGGGAATTGCCCGTCGTTTAGTACAAGATGGCGCGATTGAAGA
GCCTGCTGCTCGGGCTGCGATGGATCAGGCAGCGGCTGCGAAGGTTCCATTGCCCCAATGGTTTTCGGAAAAAAAGCTGG
TATCGGCGTCGCAACTGGCTGCTGCCAATGCAGTCGAGTTCGGTATGCCCCTGATGGATGTGTCGGCATTCGACGCAAGC
CAGAACGCAGTAAAGCTCGTCAGCGAAGAGTTGCTCCAGAAGCACCAAGTGTTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTGGGGGTGAGCAACCCAACCCAGACTCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTACGCCAGGACCCA
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCTGAAGGATTCACGCCCGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.27

0.555
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.393

99.827

0.493
  pilF Neisseria gonorrhoeae MS11

49.648

98.27

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

96.886

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446

Multiple sequence alignment