Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   GN234_RS16070 Genome accession   NZ_CP048810
Coordinates   3674741..3675805 (-) Length   354 a.a.
NCBI ID   WP_109752037.1    Uniprot ID   -
Organism   Pseudomonas bijieensis strain L22-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3669741..3680805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN234_RS16045 (GN234_15985) aroK 3670407..3670925 (-) 519 WP_003196983.1 shikimate kinase AroK -
  GN234_RS16050 (GN234_15990) pilQ 3670930..3673017 (-) 2088 WP_116833986.1 type IV pilus secretin PilQ Machinery gene
  GN234_RS16055 (GN234_15995) - 3673031..3673558 (-) 528 WP_109752040.1 pilus assembly protein PilP -
  GN234_RS16060 (GN234_16000) pilO 3673555..3674178 (-) 624 WP_109752039.1 type 4a pilus biogenesis protein PilO -
  GN234_RS16065 (GN234_16005) - 3674175..3674741 (-) 567 WP_109752038.1 PilN domain-containing protein -
  GN234_RS16070 (GN234_16010) comM 3674741..3675805 (-) 1065 WP_109752037.1 pilus assembly protein PilM Machinery gene
  GN234_RS16075 (GN234_16015) - 3676013..3678457 (+) 2445 WP_176689597.1 penicillin-binding protein 1A -
  GN234_RS16080 (GN234_16020) ltrA 3678768..3680189 (-) 1422 Protein_3212 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38067.64 Da        Isoelectric Point: 4.9070

>NTDB_id=423157 GN234_RS16070 WP_109752037.1 3674741..3675805(-) (comM) [Pseudomonas bijieensis strain L22-9]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIADLEGVGQALSRLLAKAKTPVRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=423157 GN234_RS16070 WP_109752037.1 3674741..3675805(-) (comM) [Pseudomonas bijieensis strain L22-9]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGCGAGCGATATCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGATCTCGAAGGGGTGGGCCAGGCATTGTCACGACTGTTGGCCAAGGCCAAGACGCCCGTGCGTAGCGTGGCGGTG
GCCGTTGCGGGGTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGTTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTCGAAGTGGTGGGCGTTTCGCCGC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGACGTGGAGGCTTATGCGCTGGAGCGTGCTTTCGGCTTGCTCGCCACGCAACTGGC
CGCCTCCCAGGAACGGCTGACTGTGGCGGTTATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAAATTCAGCGCCGCTATGGCTTGACGCCC
GAGCAGGCCGGCTTGGCAAAGAAGCAGGGAGGGCTGCCGGACGATTACCTGAGCGAAGTGTTGCAACCCTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGATCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTTGACCACATCCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAACAACGCCTGGGTACCCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCACCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment