Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3566_RS16250 Genome accession   NZ_CP048712
Coordinates   3804033..3805769 (-) Length   578 a.a.
NCBI ID   WP_194271719.1    Uniprot ID   -
Organism   Xanthomonas citri strain UnB-XtecTG02-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3799033..3810769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3566_RS16240 (G3566_16180) - 3800247..3801860 (+) 1614 WP_007965357.1 HAMP domain-containing sensor histidine kinase -
  G3566_RS16245 (G3566_16185) pilR 3802129..3803583 (+) 1455 WP_170922314.1 sigma-54 dependent transcriptional regulator Regulator
  G3566_RS16250 (G3566_16190) pilB 3804033..3805769 (-) 1737 WP_194271719.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G3566_RS16255 (G3566_16195) pilA2 3805811..3806224 (-) 414 WP_089111877.1 pilin Machinery gene
  G3566_RS16260 (G3566_16200) comP 3806322..3806750 (-) 429 WP_007962467.1 pilin Machinery gene
  G3566_RS16265 (G3566_16205) pilC 3807096..3808355 (+) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  G3566_RS16270 (G3566_16210) - 3808362..3809225 (+) 864 WP_003491180.1 A24 family peptidase -
  G3566_RS16275 (G3566_16215) coaE 3809239..3809850 (+) 612 WP_099802201.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62686.79 Da        Isoelectric Point: 5.7586

>NTDB_id=422477 G3566_RS16250 WP_194271719.1 3804033..3805769(-) (pilB) [Xanthomonas citri strain UnB-XtecTG02-2]
MNVAIGSNLVGITGIARRLVQDGALDEVSARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNTSLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=422477 G3566_RS16250 WP_194271719.1 3804033..3805769(-) (pilB) [Xanthomonas citri strain UnB-XtecTG02-2]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGTGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTGCCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAGCTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCATTCGACGCCAAC
CAGAACGCAGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATTAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAATACGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCAGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTTCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAGGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCAATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

51.557

100

0.516
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio cholerae strain A1552

48.09

99.654

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445

Multiple sequence alignment