Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G3566_RS14805 Genome accession   NZ_CP048712
Coordinates   3442905..3444035 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas citri strain UnB-XtecTG02-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3437905..3449035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3566_RS14780 (G3566_14720) ruvX 3438754..3439221 (-) 468 WP_003489932.1 Holliday junction resolvase RuvX -
  G3566_RS14785 (G3566_14725) - 3439214..3439780 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  G3566_RS14790 (G3566_14730) - 3440048..3440620 (+) 573 WP_089141909.1 DNA-3-methyladenine glycosylase I -
  G3566_RS14795 (G3566_14735) - 3440628..3441554 (+) 927 WP_173644273.1 DUF72 domain-containing protein -
  G3566_RS14800 (G3566_14740) - 3441729..3442403 (-) 675 WP_016851096.1 YitT family protein -
  G3566_RS14805 (G3566_14745) pilU 3442905..3444035 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G3566_RS14810 (G3566_14750) pilT 3444148..3445185 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  G3566_RS14815 (G3566_14755) - 3445596..3446288 (+) 693 WP_194271619.1 YggS family pyridoxal phosphate-dependent enzyme -
  G3566_RS14820 (G3566_14760) proC 3446332..3447186 (+) 855 WP_194271620.1 pyrroline-5-carboxylate reductase -
  G3566_RS14825 (G3566_14765) - 3447750..3448175 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  G3566_RS14830 (G3566_14770) - 3448278..3448919 (+) 642 WP_007973476.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=422472 G3566_RS14805 WP_003489921.1 3442905..3444035(-) (pilU) [Xanthomonas citri strain UnB-XtecTG02-2]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=422472 G3566_RS14805 WP_003489921.1 3442905..3444035(-) (pilU) [Xanthomonas citri strain UnB-XtecTG02-2]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATTACCCAGACCCCGCTGACCGCGCAGCAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTGAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGTAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGTTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTATTGATGGATCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGCCGCAGCCGGCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCTTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGATGCGTTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGTGTAGAGATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment