Detailed information NTG0422201

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LYSZa7_RS29195
• Genome accession: NZ_CP061699
• Coordinates: 6307154..6308302 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain LYSZa7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6302154..6313302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LYSZa7_RS29175 (LYSZa7_29090)	-	6302892..6304076 (-)	1185	WP_033955000.1	cystathionine gamma-synthase	-
LYSZa7_RS29180 (LYSZa7_29095)	-	6304073..6305446 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
LYSZa7_RS29185 (LYSZa7_29100)	-	6305689..6306093 (-)	405	WP_003084560.1	NINE protein	-
LYSZa7_RS29190 (LYSZa7_29105)	czcD	6306247..6307146 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LYSZa7_RS29195 (LYSZa7_29110)	pilU	6307154..6308302 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
LYSZa7_RS29200 (LYSZa7_29115)	pilT	6308480..6309514 (-)	1035	WP_034028650.1	type IV pilus twitching motility protein PilT	Machinery gene
LYSZa7_RS29205 (LYSZa7_29120)	-	6309729..6310421 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LYSZa7_RS29210 (LYSZa7_29125)	proC	6310433..6311254 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LYSZa7_RS29215 (LYSZa7_29130)	-	6311265..6311858 (+)	594	WP_003084544.1	YggT family protein	-
LYSZa7_RS29220 (LYSZa7_29135)	-	6311855..6312151 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=422201 LYSZa7_RS29195 WP_003110016.1 6307154..6308302(-) (pilU) [Pseudomonas aeruginosa strain LYSZa7]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=422201 LYSZa7_RS29195 WP_003110016.1 6307154..6308302(-) (pilU) [Pseudomonas aeruginosa strain LYSZa7]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382