Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GYM47_RS09090 Genome accession   NZ_CP048602
Coordinates   1973928..1975685 (+) Length   585 a.a.
NCBI ID   WP_153843351.1    Uniprot ID   -
Organism   Vreelandella piezotolerans strain NBT06E8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1968928..1980685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM47_RS09060 (GYM47_09025) - 1970186..1970914 (+) 729 WP_153843347.1 sulfite exporter TauE/SafE family protein -
  GYM47_RS09065 (GYM47_09030) - 1971038..1971238 (+) 201 WP_139527470.1 hypothetical protein -
  GYM47_RS09075 (GYM47_09040) - 1971808..1972620 (-) 813 WP_153843348.1 inositol monophosphatase family protein -
  GYM47_RS09080 (GYM47_09045) - 1972686..1973135 (-) 450 WP_153843349.1 anti-virulence regulator CigR family protein -
  GYM47_RS18475 (GYM47_09050) - 1973292..1973696 (-) 405 WP_153843350.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GYM47_RS09090 (GYM47_09055) pilB 1973928..1975685 (+) 1758 WP_153843351.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GYM47_RS09095 (GYM47_09060) - 1975694..1976926 (+) 1233 WP_153843352.1 type II secretion system F family protein -
  GYM47_RS09100 (GYM47_09065) - 1976956..1977801 (+) 846 WP_153843353.1 A24 family peptidase -
  GYM47_RS09105 (GYM47_09070) coaE 1977834..1978430 (+) 597 WP_153843354.1 dephospho-CoA kinase -
  GYM47_RS09110 (GYM47_09075) yacG 1978445..1978678 (+) 234 WP_139527455.1 DNA gyrase inhibitor YacG -
  GYM47_RS09115 (GYM47_09080) - 1978694..1979563 (+) 870 WP_139527751.1 DNA replication terminus site-binding protein -
  GYM47_RS09120 (GYM47_09085) - 1979540..1979902 (-) 363 WP_153843355.1 hypothetical protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 63884.14 Da        Isoelectric Point: 6.0410

>NTDB_id=421727 GYM47_RS09090 WP_153843351.1 1973928..1975685(+) (pilB) [Vreelandella piezotolerans strain NBT06E8]
MSQTVPESTLSHAALNGGLRGIAKRLVKHGLLTDAQATVAETTAAELEISLLQHVIDSGLVNADAAAVAAAWEYGLPVVD
LEAIRVSALPPASDYPVKILERLDILPLARHGHRLTVAVPYPATLTQLDELQFATGLSVDGVLAPADQLRNTLVQYLAQN
ERSMLDELENIDDAVSALNIVQHADGDEVELDKAAQNSDDAPVVKFVNKILLDAISRGASDIHFEPYETQYRVRFRIDGM
LVEIARPPFALRHRIAARLKIMSRLDISERRLPQDGALKLQLSRTRSIDFRVNSLPTVWGEKVVLRILDPTSAQLGIDQL
GFSPEQQAAYEYALKKPQGMILVTGPTGSGKTVTLYTGINILNQVQRNICTAEDPVEIKVSGINQVNVLPKIGLNFASAL
RAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVATYNIASSVSLIIAQRLARKLCNQ
CKTPADIPAEALRKAGFTQQDIRNATIYKAVGCKQCTQGYKGRLGVYEVVPITDDMRQLIMRDGNAIEIDQQARREGYPS
LYQSCLSRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=421727 GYM47_RS09090 WP_153843351.1 1973928..1975685(+) (pilB) [Vreelandella piezotolerans strain NBT06E8]
ATGAGCCAAACCGTCCCAGAGTCGACGCTTAGCCATGCGGCCCTCAATGGAGGGTTGCGAGGCATTGCTAAGCGCCTGGT
CAAGCATGGTCTTTTAACGGATGCGCAAGCGACCGTTGCAGAGACCACGGCGGCTGAACTCGAGATATCGCTACTGCAAC
ATGTCATCGATAGTGGCCTGGTCAATGCGGATGCCGCAGCGGTGGCGGCTGCGTGGGAATATGGCTTGCCGGTGGTGGAT
CTCGAAGCCATACGTGTCAGCGCACTACCGCCTGCCAGCGACTACCCCGTCAAAATACTCGAGCGGCTGGATATTTTGCC
TCTGGCTCGTCACGGTCACCGCCTGACCGTCGCCGTACCCTACCCGGCCACATTGACCCAGCTCGATGAGCTGCAGTTCG
CCACAGGGCTGAGTGTGGATGGCGTTCTGGCGCCTGCCGATCAACTGCGCAATACGCTAGTGCAGTACCTAGCGCAGAAC
GAGCGCAGCATGCTCGATGAGCTGGAAAATATCGATGATGCCGTCAGCGCGCTCAATATCGTTCAGCACGCTGATGGAGA
CGAGGTCGAACTCGACAAAGCCGCTCAAAATAGCGATGACGCGCCAGTCGTTAAATTCGTCAATAAAATCCTGCTCGATG
CGATCAGTCGAGGCGCGTCGGACATTCATTTTGAGCCCTACGAAACGCAGTACCGGGTGCGTTTCCGTATCGATGGCATG
TTGGTCGAAATCGCGCGACCACCTTTTGCGCTGCGCCACCGCATTGCGGCCCGCTTGAAGATCATGTCTCGACTGGATAT
TTCAGAGCGCCGCTTGCCACAAGATGGCGCCTTGAAGCTGCAACTGTCGCGGACCCGCTCTATCGACTTTCGGGTGAACT
CGCTACCCACGGTATGGGGGGAAAAAGTCGTACTGCGGATTTTGGATCCTACGTCCGCTCAGCTCGGGATCGACCAGCTC
GGCTTTAGTCCTGAACAGCAGGCCGCCTATGAGTATGCGTTGAAAAAGCCCCAAGGAATGATTTTGGTGACGGGACCAAC
GGGTAGCGGTAAAACGGTGACGCTCTACACCGGCATCAATATCTTGAACCAAGTTCAACGCAATATTTGCACCGCTGAAG
ATCCGGTCGAAATCAAAGTATCGGGCATCAACCAGGTCAACGTGCTGCCTAAAATCGGGCTCAATTTTGCCAGTGCGCTG
CGCGCATTTCTGCGCCAAGACCCAGATGTGGTCATGGTGGGGGAAATTCGCGATTTGGAAACCGCAGAAATTGCGGTCAA
AGCGGCCCAAACCGGCCACCTGGTGCTCTCTACCGTGCATACCAACTCGGCGGCGGAAACGCTGACCCGTCTCGCGAACA
TGGGGGTGGCTACCTATAACATCGCCAGCTCGGTCAGCCTGATCATTGCACAGCGACTAGCACGCAAACTGTGTAATCAG
TGTAAAACACCAGCCGACATTCCGGCGGAGGCGCTTCGTAAAGCAGGCTTCACTCAGCAAGATATCCGCAACGCGACGAT
CTATAAAGCGGTGGGCTGCAAGCAGTGCACCCAGGGGTACAAAGGGCGTTTAGGCGTTTACGAGGTGGTGCCGATTACCG
ATGACATGCGCCAACTGATCATGCGCGATGGCAACGCTATCGAGATCGACCAGCAGGCACGCCGCGAAGGTTACCCTAGC
CTGTATCAGAGCTGCTTGAGCCGAGTTCTGGATGGCAGCACCAGCCTGGAAGAAGTCAATCGTATCAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-161)

Type II/IV secretion system protein (T2SSE)

(209-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.357

96.752

0.516
  pilB Acinetobacter baumannii D1279779

50.88

97.094

0.494
  pilB Acinetobacter baylyi ADP1

49.567

98.632

0.489
  pilB Vibrio cholerae strain A1552

49.819

94.359

0.47
  pilB Vibrio campbellii strain DS40M4

47.11

97.607

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

47.425

96.239

0.456
  pilF Neisseria gonorrhoeae MS11

50.837

81.709

0.415

Multiple sequence alignment