Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIFE_RS13735 Genome accession   NC_015942
Coordinates   2828332..2830041 (-) Length   569 a.a.
NCBI ID   WP_014030178.1    Uniprot ID   G0JT29
Organism   Acidithiobacillus ferrivorans SS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2823332..2835041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFE_RS13715 (Acife_2857) coaE 2823855..2824457 (-) 603 WP_014030174.1 dephospho-CoA kinase -
  ACIFE_RS13720 (Acife_2858) - 2824458..2825258 (-) 801 WP_041649997.1 prepilin peptidase -
  ACIFE_RS13725 (Acife_2859) pilR 2825255..2826613 (-) 1359 WP_014030176.1 sigma-54-dependent transcriptional regulator Regulator
  ACIFE_RS13730 (Acife_2860) - 2826610..2828208 (-) 1599 WP_014030177.1 two-component system sensor histidine kinase NtrB -
  ACIFE_RS13735 (Acife_2861) pilB 2828332..2830041 (-) 1710 WP_014030178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIFE_RS13740 (Acife_2862) aroE 2830011..2830910 (-) 900 WP_014030179.1 shikimate dehydrogenase -
  ACIFE_RS13745 (Acife_2863) - 2830888..2832051 (-) 1164 WP_041650000.1 anhydro-N-acetylmuramic acid kinase -
  ACIFE_RS13750 (Acife_2864) - 2832119..2832607 (-) 489 WP_014030181.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ACIFE_RS13755 (Acife_2865) hemH 2832670..2833683 (-) 1014 WP_014030182.1 ferrochelatase -
  ACIFE_RS13760 (Acife_2866) xth 2833680..2834453 (-) 774 WP_014030183.1 exodeoxyribonuclease III -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62646.41 Da        Isoelectric Point: 6.2050

>NTDB_id=42134 ACIFE_RS13735 WP_014030178.1 2828332..2830041(-) (pilB) [Acidithiobacillus ferrivorans SS3]
MATNPAHIALTPVLRALVSNGLSDEAQLQSLAADPARGKMPLLFYAVEKGAVPASVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAAANSMQGGIDDVFMD
KPRGEEEQEEFDLAQESDGSVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFMPDQRKAFEEAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLSFAAALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPEQALIEAGF
APEDLQGWRPLGAAGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQATQEGVLTMRQNGLRRIREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=42134 ACIFE_RS13735 WP_014030178.1 2828332..2830041(-) (pilB) [Acidithiobacillus ferrivorans SS3]
ATGGCTACAAATCCAGCACACATCGCGTTGACGCCCGTATTGCGGGCATTAGTCAGCAACGGGCTAAGCGATGAGGCGCA
ATTGCAAAGCCTTGCCGCTGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTATGCGGTTGAGAAGGGCGCCGTTCCTG
CCTCGGTGCTGATGGCGCACCTTTCTGCGCGCTATAACATGCCCATGCTCGACCTGGATGCGGTCATGATTAATGAGTTG
CTCATCCGGAAGCTCGACAAGGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGATACCCTCTACCT
CGCCATGGCAGATCCTACGGATTTTAAGGCGGTGGAGGATGTGAAGTTCAACACCGGCCTGCAGGTGTTGCCGATACTGG
TCGAGGCCAATAAGCTGGTAAAGGCGGTGAATGCCGCGGCCAACAGTATGCAAGGCGGGATTGACGATGTTTTCATGGAT
AAGCCGCGCGGCGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGAAAGTGATGGCAGTGTCGAGGATGCGCCGGTGGT
GCGTTTTGCCCGGCAATTGCTGCTGGATGCCATTCAACGCGGGGTTTCCGATATTCATCTGGAACCCTATGAAAAGGATT
TTCGGGTGCGTTACCGCCTCGATGGCGTATTGCAGGATGTGCTGCATCCGCCGGTGGCGTTGCGTGACGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGCCTGGATATTTCTGAGCGGCGTTTACCCCAGGATGGCCGTCTGCGGGTCAGGGTGCCACC
GGCGCGGGTTATTGATTTCCGGGTCTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAATTGGGCTTTATGCCGGACCAGCGCAAAGCTTTTGAAGAGGCTATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACAACGACCCTCTACACCGCGCTGAATATTCTCAACAC
CGGCGACTGCAACATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTATACGGGATCAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAGCTTTGCCGCCGCCTTGCGTTCCTTCCTGCGCCAGGACCCTGACATTATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
ACCACAGAGTCTCACCCGTCTGGAAAATATGGGTATCCCCACCTATAACATAGCGGGCGGTGTGCACCTGGTGATGGCGC
AGCGACTTGCCCGCAAACTCTGCCCGCACTGTAAAAAGCCGCTGCGCATTCCCGAACAGGCCCTGATTGAGGCGGGATTT
GCGCCCGAGGATCTGCAGGGCTGGCGTCCTCTGGGCGCGGCGGGCTGTGATCAGTGCAATAACACCGGGTATAAAGGTCG
GATGGGACTCTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGTGGTGGTACTTCCATCGATCTGG
CACGGCAGGCGACGCAGGAGGGAGTGCTGACCATGCGGCAAAACGGATTGAGGCGGATCAGGGAGGGCGTCACTAGTCTT
GAAGAGGTCCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0JT29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.007

100

0.487
  pilB Acinetobacter baumannii D1279779

47.711

99.824

0.476
  pilB Acinetobacter baylyi ADP1

46.235

100

0.464
  pilF Neisseria gonorrhoeae MS11

45.276

98.594

0.446
  pilB Vibrio parahaemolyticus RIMD 2210633

43.63

100

0.439
  pilB Vibrio cholerae strain A1552

47.025

91.564

0.431
  pilB Vibrio campbellii strain DS40M4

44.762

92.267

0.413
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.322

0.371

Multiple sequence alignment