Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GZH78_RS03105 Genome accession   NZ_CP048408
Coordinates   687046..688110 (+) Length   354 a.a.
NCBI ID   WP_039766420.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 682046..693110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS03095 (GZH78_03095) - 682759..684027 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  GZH78_RS03100 (GZH78_03100) - 684381..686828 (-) 2448 WP_039766412.1 penicillin-binding protein 1A -
  GZH78_RS03105 (GZH78_03105) pilM 687046..688110 (+) 1065 WP_039766420.1 pilus assembly protein PilM Machinery gene
  GZH78_RS03110 (GZH78_03110) - 688110..688676 (+) 567 WP_074689559.1 PilN domain-containing protein -
  GZH78_RS03115 (GZH78_03115) pilO 688673..689296 (+) 624 WP_074689561.1 type 4a pilus biogenesis protein PilO -
  GZH78_RS03120 (GZH78_03120) - 689293..689817 (+) 525 WP_074689563.1 pilus assembly protein PilP -
  GZH78_RS03125 (GZH78_03125) pilQ 689832..691910 (+) 2079 WP_039766429.1 type IV pilus secretin PilQ Machinery gene
  GZH78_RS03130 (GZH78_03130) aroK 691915..692433 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38101.59 Da        Isoelectric Point: 4.6857

>NTDB_id=421106 GZH78_RS03105 WP_039766420.1 687046..688110(+) (pilM) [Pseudomonas fluorescens strain DR397]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVTGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=421106 GZH78_RS03105 WP_039766420.1 687046..688110(+) (pilM) [Pseudomonas fluorescens strain DR397]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAGCT
GAGTCGTCAGGGCGAGCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCGTTGCCGGCGAGTGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTCGGCCAGGCCCTGTCCCGGGTGCTGGTCAAGGCGCGTACCGGGTTGAAAACCGTGGCGGTG
GCCGTGGCCGGCTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGATGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTACCCGCTGGACGAGGTCGCCATCGACTTTGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAGCGGGTCAACGTACTGCTGGCCGCCTGTCGCAAGGAAAACGTGGAAGTCCGCGAAGCGGCGCTCGCTCTC
GCCGGGCTGACCGCGCGGGTGGTAGATGTAGAGGCGTATGCGCTGGAGCGCTCGTTCGGTCTGCTGGCCACGCAACTGGC
TGCGTCCCAGGATCGGCTGACCGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAATGGCC
GGATCATCTACACACGCGAACAGCTGTTCGGCGGTCGGCAGTTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCCTT
GAACAGGCGGGACTTGCCAAGAAGCAGGGCGGCCTGCCGGACGATTACGTCAGCGAGGTGCTGCAGCCATTTCGTGAGGC
GCTGGTGCAGCAGGTGTCACGCTCCCTGCAGTTCTTTTTCGCGTCCGGCCAGTACAACGCCGTCGACCACATTCTGCTGG
CGGGCGGCACGGCATCGGTGACGGGGCTCGACCGGCTGATCGAAGAGCGCCTGAACACTCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAAGTCAACGCCGGGGCTTTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.296

100

0.494

Multiple sequence alignment