Detailed information NTG0420133

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: IBZ91_RS03620
• Genome accession: NZ_CP061108
• Coordinates: 756626..757285 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain 1EC187
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 751626..762285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IBZ91_RS03595 (IBZ91_03590)	ygiN	752527..752841 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
IBZ91_RS03600 (IBZ91_03595)	mdaB	752872..753453 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
IBZ91_RS24500	-	753703..754182 (+)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
IBZ91_RS24505	-	754185..754895 (+)	711	WP_000834030.1	hypothetical protein	-
IBZ91_RS03610 (IBZ91_03605)	ygiZ	754902..755234 (+)	333	WP_000914690.1	DUF2645 family protein	-
IBZ91_RS03615 (IBZ91_03610)	qseC	755280..756629 (-)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
IBZ91_RS03620 (IBZ91_03615)	ciaR	756626..757285 (-)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
IBZ91_RS03625 (IBZ91_03620)	ygiW	757437..757829 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
IBZ91_RS03630 (IBZ91_03625)	ygiV	757882..758364 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
IBZ91_RS03635 (IBZ91_03630)	mqsR	758569..758865 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
IBZ91_RS03640 (IBZ91_03635)	mqsA	758867..759262 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
IBZ91_RS03645 (IBZ91_03640)	ygiS	759395..761002 (+)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=420133 IBZ91_RS03620 WP_001221502.1 756626..757285(-) (ciaR) [Escherichia coli strain 1EC187]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=420133 IBZ91_RS03620 WP_001221502.1 756626..757285(-) (ciaR) [Escherichia coli strain 1EC187]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365