Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   IB009_RS02230 Genome accession   NZ_CP061101
Coordinates   470050..471174 (+) Length   374 a.a.
NCBI ID   WP_000228519.1    Uniprot ID   -
Organism   Escherichia coli strain 1EC213     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 465050..476174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IB009_RS02205 (IB009_02200) mscL 465176..465586 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  IB009_RS02210 (IB009_02205) trkA 465716..467092 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  IB009_RS02215 (IB009_02210) rsmB 467114..468403 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  IB009_RS02220 (IB009_02215) fmt 468449..469396 (-) 948 WP_000004432.1 methionyl-tRNA formyltransferase -
  IB009_RS02225 (IB009_02220) def 469411..469920 (-) 510 WP_000114984.1 peptide deformylase -
  IB009_RS02230 (IB009_02225) dprA 470050..471174 (+) 1125 WP_000228519.1 DNA-protecting protein DprA Machinery gene
  IB009_RS02235 (IB009_02230) smg 471146..471619 (+) 474 WP_000460670.1 DUF494 family protein Smg -
  IB009_RS02240 (IB009_02235) yrdD 471648..472190 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  IB009_RS02245 (IB009_02240) tsaC 472195..472767 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  IB009_RS02250 (IB009_02245) aroE 472772..473590 (+) 819 WP_000451238.1 shikimate dehydrogenase -
  IB009_RS02255 (IB009_02250) yrdB 473587..473844 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  IB009_RS02260 (IB009_02255) yrdA 473820..474374 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41006.95 Da        Isoelectric Point: 6.3839

>NTDB_id=420049 IB009_RS02230 WP_000228519.1 470050..471174(+) (dprA) [Escherichia coli strain 1EC213]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLQATTDYPGALFVEGELHALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=420049 IB009_RS02230 WP_000228519.1 470050..471174(+) (dprA) [Escherichia coli strain 1EC213]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACTGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCAGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCATAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACAAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTTTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTAGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

45.104

90.107

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393