Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSTAB_RS04800 Genome accession   NC_015740
Coordinates   1024086..1025789 (+) Length   567 a.a.
NCBI ID   WP_013981942.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019086..1030789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSTAB_RS21085 - 1021039..1021593 (-) 555 WP_080564968.1 REP-associated tyrosine transposase -
  PSTAB_RS21505 - 1022846..1023187 (-) 342 WP_148263409.1 hypothetical protein -
  PSTAB_RS21090 - 1023297..1023776 (-) 480 WP_041771654.1 pilin -
  PSTAB_RS04800 (PSTAB_0957) pilB 1024086..1025789 (+) 1704 WP_013981942.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSTAB_RS04805 (PSTAB_0958) pilC 1025791..1027008 (+) 1218 WP_013981943.1 type II secretion system F family protein Machinery gene
  PSTAB_RS04810 (PSTAB_0959) pilD 1027011..1027880 (+) 870 WP_013981944.1 prepilin peptidase Machinery gene
  PSTAB_RS21095 (PSTAB_0960) coaE 1028011..1028619 (+) 609 WP_013981945.1 dephospho-CoA kinase -
  PSTAB_RS04820 yacG 1028616..1028813 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  PSTAB_RS04825 (PSTAB_0961) - 1028826..1029506 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  PSTAB_RS04830 (PSTAB_0962) - 1029563..1030603 (-) 1041 WP_013981947.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61895.94 Da        Isoelectric Point: 5.3572

>NTDB_id=41740 PSTAB_RS04800 WP_013981942.1 1024086..1025789(+) (pilB) [Stutzerimonas stutzeri]
MSENISLSGLARQMVVAGVLDEKTAQQAQAQAVRGKTPFVTYVVQNKLAKGRAIAELASDQFGVALLDLSALNKESLPKD
LISEKLARQHRVLPLSKRGTKLYVAISDPANHQSIADIQFSTGLSIESILVEDDKLGEAIDKLYESGATGLEELGEVDLD
GVDVESVTEDGKAEEGGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=41740 PSTAB_RS04800 WP_013981942.1 1024086..1025789(+) (pilB) [Stutzerimonas stutzeri]
ATGAGCGAAAACATTTCCCTATCCGGGCTGGCACGGCAGATGGTCGTGGCCGGCGTGTTGGACGAAAAAACGGCCCAGCA
GGCGCAGGCTCAAGCCGTTCGCGGCAAAACACCCTTTGTCACCTATGTAGTGCAGAACAAGTTGGCCAAAGGCCGCGCCA
TAGCCGAGCTTGCTTCCGATCAATTCGGTGTAGCGCTGCTGGACCTGTCTGCGCTGAACAAGGAGTCGCTGCCCAAGGAT
CTGATCAGTGAGAAGTTGGCCCGCCAACATCGGGTGCTGCCGCTTTCCAAGCGCGGCACCAAGCTTTATGTCGCCATCTC
CGATCCGGCTAATCATCAATCGATTGCCGATATCCAGTTCAGTACCGGGCTGAGCATCGAGTCGATTCTGGTGGAAGACG
ACAAGCTGGGCGAGGCCATCGACAAACTGTACGAAAGCGGCGCCACCGGACTAGAGGAGCTGGGCGAAGTCGATCTGGAT
GGGGTCGATGTCGAGTCCGTCACCGAAGATGGAAAAGCTGAGGAAGGCGGAGAGGCTGCTGACGACGCCCCTGTAGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGTGGCTCGTCCGATTTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTGCGGCTTCGCACGGATGGCATCCTGCATGAGGTCGCGCGTCCGCCGATTCAGCTTGCACCGCGAATTTCTGCGCGC
CTAAAGGTCATGGCCGGGCTGGATATCTCCGAGCGGCGTAAGCCGCAGGATGGCCGGATCAAGATGAAGCTGTCTAAGAC
CAAGGCCATCGATTTCCGCGTAAATACGCTACCCACCCTATGGGGCGAGAAAATCGTGATGCGTATCCTCGACCCGTCCA
GTGCGCAGATGGGGATCGATGCCCTGGGTTACGAGGAAGAACAGAAAGAGCTCTACATGAATGCCTTGCGCCAGCCACAA
GGCATGATCCTGGTTACCGGCCCGACTGGGTCGGGCAAGACGGTTTCGCTGTATACCGGTCTGAATATCCTCAATACCGT
GGACGTGAACATCTCCACCGCGGAAGACCCAGTGGAGATCAATCTGGAGGGTATTAACCAGGTCAACGTAAACCCGCGCC
AAGGTATGGACTTCGCTCAGGCCCTCCGTGCCTTTCTACGTCAGGACCCGGACATCATTATGGTGGGCGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGTAATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAATATCGCGACCTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCGCGAAAGCTTTGCAATAGCTGCAAGAAAGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAACCTTCAAGATCTATGGTCCGGTTGGTTGCGACAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCGTTGGCGAGGATTATCATGGAGGACGGCAATTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTACTTCGGCCCTAGTGAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTGAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.752

99.471

0.594
  pilB Acinetobacter baylyi ADP1

59.503

99.295

0.591
  pilB Legionella pneumophila strain ERS1305867

54.737

100

0.55
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

52.308

91.711

0.48
  pilB Vibrio cholerae strain A1552

53.021

90.476

0.48
  pilF Neisseria gonorrhoeae MS11

48.135

99.295

0.478
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.266

96.12

0.377

Multiple sequence alignment