Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   HUG14_RS01755 Genome accession   NZ_CP060580
Coordinates   372478..374361 (-) Length   627 a.a.
NCBI ID   WP_058211607.1    Uniprot ID   -
Organism   Lactococcus lactis strain K_LL005     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 367478..379361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUG14_RS01725 (HUG14_01725) - 367884..368564 (-) 681 WP_012898438.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  HUG14_RS01730 (HUG14_01730) macP 368596..368874 (-) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  HUG14_RS01735 (HUG14_01735) - 368867..369451 (-) 585 WP_033899834.1 NUDIX hydrolase -
  HUG14_RS01740 (HUG14_01740) glmU 369556..370932 (-) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  HUG14_RS01745 (HUG14_01745) proC 371160..371948 (+) 789 WP_033899830.1 pyrroline-5-carboxylate reductase -
  HUG14_RS01750 (HUG14_01750) rpsO 372027..372296 (-) 270 WP_015427039.1 30S ribosomal protein S15 -
  HUG14_RS01755 (HUG14_01755) pknB 372478..374361 (-) 1884 WP_058211607.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  HUG14_RS01760 (HUG14_01760) - 374361..375137 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  HUG14_RS01765 (HUG14_01765) rsmB 375218..376492 (-) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  HUG14_RS01770 (HUG14_01770) - 376691..377197 (-) 507 WP_012898444.1 Asp23/Gls24 family envelope stress response protein -
  HUG14_RS01775 (HUG14_01775) - 377218..377406 (-) 189 WP_033899826.1 DUF2273 domain-containing protein -
  HUG14_RS01780 (HUG14_01780) amaP 377417..377977 (-) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  HUG14_RS01785 (HUG14_01785) - 378005..378247 (-) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67948.01 Da        Isoelectric Point: 4.7401

>NTDB_id=415305 HUG14_RS01755 WP_058211607.1 372478..374361(-) (pknB) [Lactococcus lactis strain K_LL005]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=415305 HUG14_RS01755 WP_058211607.1 372478..374361(-) (pknB) [Lactococcus lactis strain K_LL005]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGACCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTGTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-265)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(434-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.081

86.762

0.426
  stkP/pknB Streptococcus salivarius strain HSISS4

51.252

82.775

0.424
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384