Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GU334_RS10615 Genome accession   NZ_CP047628
Coordinates   2163026..2164111 (-) Length   361 a.a.
NCBI ID   WP_245325542.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain Lr_19_14     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2158026..2169111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU334_RS10570 (GU334_10530) - 2158223..2158993 (-) 771 WP_244165990.1 metal ABC transporter ATP-binding protein -
  GU334_RS10575 (GU334_10535) - 2158993..2159442 (-) 450 WP_096039675.1 zinc-dependent MarR family transcriptional regulator -
  GU334_RS10580 (GU334_10540) - 2159518..2160366 (-) 849 WP_167838309.1 aminotransferase class IV -
  GU334_RS10585 (GU334_10545) ispE 2160450..2161301 (-) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  GU334_RS10590 (GU334_10550) comGG 2161306..2161590 (-) 285 WP_096039677.1 competence type IV pilus minor pilin ComGG -
  GU334_RS10595 (GU334_10555) - 2161602..2161874 (-) 273 WP_303620936.1 competence type IV pilus minor pilin ComGF -
  GU334_RS10600 (GU334_10560) comGE 2162020..2162325 (-) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  GU334_RS11995 comGD 2162297..2162782 (-) 486 WP_312186085.1 competence type IV pilus minor pilin ComGD -
  GU334_RS10610 (GU334_10570) comYC 2162697..2163017 (-) 321 WP_061774318.1 competence type IV pilus major pilin ComGC Machinery gene
  GU334_RS10615 (GU334_10575) comYB 2163026..2164111 (-) 1086 WP_245325542.1 competence type IV pilus assembly protein ComGB Machinery gene
  GU334_RS10620 (GU334_10580) comGA/cglA/cilD 2163993..2164934 (-) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  GU334_RS10625 (GU334_10585) - 2164997..2166841 (-) 1845 WP_096039681.1 acyltransferase family protein -
  GU334_RS10630 (GU334_10590) - 2166838..2167200 (-) 363 WP_061774315.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 41422.21 Da        Isoelectric Point: 10.0964

>NTDB_id=414711 GU334_RS10615 WP_245325542.1 2163026..2164111(-) (comYB) [Lactococcus raffinolactis strain Lr_19_14]
MLKKHSKGMPTTSGMPKLKNWLQKDISHLIRRQPKRLAIKQQVKLMQLMNNLFLSGFHLAEIVNFLSRSKLVEERFVATM
RDGLSNGQNLSQILNDLRFSNLVVTQVALADYHGDTQQTLTLIIDNLTKIQKVRKKLVAVSTYPIILLTFLIIIVLGLKT
YLLPQVETDSFAGTVINALPLIFIGLSLVLTFFALGLIRYFKSTSPLANVQWLGRLPLIRSYLKLYLTAFYAREWGNLVK
QGLEMRQTLEMMTSQQNLLFREIGQDIRRAMQVGEEFHDKIKQYSFFTPELALIIEYGEMKGKLGDELLIYSDESWTKFF
EKVESAMNVIQPLVFLFVALMIVLIYAAMLLPIYAQMDLGM

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=414711 GU334_RS10615 WP_245325542.1 2163026..2164111(-) (comYB) [Lactococcus raffinolactis strain Lr_19_14]
GTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAAAATTGGTTACAGAAGGACATCTC
ACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAAGCAACAAGTGAAGCTGATGCAGTTGATGAATAACCTCTTTT
TGAGTGGTTTTCATTTAGCAGAAATTGTTAATTTTTTGAGCCGCTCGAAGCTAGTGGAGGAGAGATTTGTTGCGACTATG
CGAGATGGCTTATCGAATGGTCAAAATCTCTCACAAATCTTAAATGATTTACGTTTTTCAAACTTAGTCGTCACACAAGT
TGCTTTAGCAGATTACCATGGTGATACCCAGCAAACACTGACATTAATAATAGATAATTTAACAAAAATTCAGAAGGTTC
GAAAAAAATTGGTGGCTGTATCGACCTATCCCATCATCTTGTTAACTTTTCTAATCATCATCGTACTGGGATTAAAAACC
TATTTGCTACCCCAAGTTGAAACGGATAGTTTTGCGGGCACTGTGATCAATGCCTTACCTTTGATTTTTATCGGGTTGAG
CCTCGTGTTAACTTTTTTTGCGTTAGGACTGATTCGGTATTTCAAATCAACATCACCATTGGCTAATGTCCAATGGTTAG
GTCGTCTTCCCTTGATCCGATCGTACTTAAAGCTTTATCTGACAGCGTTTTATGCGCGAGAGTGGGGAAACTTGGTTAAG
CAAGGTTTGGAGATGCGACAAACATTGGAGATGATGACCAGCCAACAGAATCTACTTTTTCGTGAAATTGGTCAAGATAT
AAGGCGGGCTATGCAAGTGGGGGAAGAATTTCACGACAAGATTAAGCAATACAGCTTTTTCACACCGGAGTTGGCCCTCA
TCATCGAATATGGTGAAATGAAGGGCAAATTAGGTGATGAATTACTCATCTATTCGGATGAATCCTGGACAAAATTTTTT
GAAAAGGTTGAAAGCGCCATGAATGTCATTCAGCCATTGGTTTTTTTGTTTGTCGCTTTGATGATTGTTTTAATCTATGC
AGCTATGTTGCTACCTATTTATGCTCAGATGGATCTGGGCATGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(47-164)

Type II secretion system (T2SS), protein F (T2SSF)

(232-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

50.435

95.568

0.482
  comYB Streptococcus mutans UA159

50.435

95.568

0.482
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

94.183

0.474
  comGB Lactococcus lactis subsp. cremoris KW2

48.974

94.46

0.463
  comGB/cglB Streptococcus pneumoniae R6

46.866

92.798

0.435
  comGB/cglB Streptococcus pneumoniae TIGR4

46.866

92.798

0.435
  comGB/cglB Streptococcus mitis NCTC 12261

46.866

92.798

0.435
  comGB/cglB Streptococcus pneumoniae Rx1

46.866

92.798

0.435
  comGB/cglB Streptococcus pneumoniae D39

46.866

92.798

0.435
  comGB/cglB Streptococcus mitis SK321

46.269

92.798

0.429

Multiple sequence alignment