Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GU336_RS01530 Genome accession   NZ_CP047616
Coordinates   296746..297831 (+) Length   361 a.a.
NCBI ID   WP_310592697.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain Lr_19_5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291746..302831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU336_RS01510 (GU336_01510) - 292590..293497 (-) 908 Protein_285 IS30 family transposase -
  GU336_RS01515 (GU336_01515) - 293657..294019 (+) 363 WP_061774315.1 hypothetical protein -
  GU336_RS01520 (GU336_01520) - 294016..295860 (+) 1845 WP_167838307.1 acyltransferase family protein -
  GU336_RS01525 (GU336_01525) comGA/cglA/cilD 295923..296864 (+) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  GU336_RS01530 (GU336_01530) comYB 296746..297831 (+) 1086 WP_310592697.1 competence type IV pilus assembly protein ComGB Machinery gene
  GU336_RS01535 (GU336_01535) comYC 297840..298160 (+) 321 WP_061774318.1 competence type IV pilus major pilin ComGC Machinery gene
  GU336_RS01540 (GU336_01540) comGD 298111..298560 (+) 450 WP_167838308.1 competence type IV pilus minor pilin ComGD -
  GU336_RS01545 (GU336_01545) comGE 298532..298837 (+) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  GU336_RS01550 (GU336_01550) comGF 298812..299255 (+) 444 WP_244165991.1 competence type IV pilus minor pilin ComGF -
  GU336_RS01555 (GU336_01555) comGG 299267..299551 (+) 285 WP_096039677.1 competence type IV pilus minor pilin ComGG -
  GU336_RS01560 (GU336_01560) ispE 299556..300407 (+) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  GU336_RS01565 (GU336_01565) - 300491..301339 (+) 849 WP_167838309.1 aminotransferase class IV -
  GU336_RS01570 (GU336_01570) - 301415..301864 (+) 450 WP_061774324.1 zinc-dependent MarR family transcriptional regulator -
  GU336_RS01575 (GU336_01575) - 301864..302634 (+) 771 WP_244165990.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 41434.26 Da        Isoelectric Point: 10.0964

>NTDB_id=414627 GU336_RS01530 WP_310592697.1 296746..297831(+) (comYB) [Lactococcus raffinolactis strain Lr_19_5]
MLKKHSKGMPTTSGMPKLKNWLQKDISHLIRRQPKRLAIKQQVKLMQLMNNLFLSGFHLAEIVNFLSRSKLVEERFVATM
RDGLSNGQNLSQILNDLRFSNLVVTQVALADYHGDTQQTLTLIIDNLTKIQKVRKKLVAVSTYPIILLTFLIIIVLGLKT
YLLPQVETDSFAGTVINALPLIFIGLSLVLTFFALGLIRYFKSTSPLANVQWLGRLPLIRSYLKLYLTAFYAREWGNLVK
QGLEMRQILEMMTSQQNLLFREIGQDIRRAMQVGEEFHDKIKQYSFFTPELALIIEYGEMKGKLGDELLIYSDESWTKFF
EKVESAMNVIQPLVFLFVALMIVLIYAAMLLPIYAQMDLGM

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=414627 GU336_RS01530 WP_310592697.1 296746..297831(+) (comYB) [Lactococcus raffinolactis strain Lr_19_5]
GTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAAAATTGGTTACAGAAGGACATCTC
ACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAAGCAACAAGTGAAGCTGATGCAGTTGATGAATAACCTCTTTT
TGAGTGGTTTTCATTTAGCAGAAATTGTTAATTTTTTGAGCCGCTCGAAGCTAGTGGAGGAGAGATTTGTTGCGACTATG
CGAGATGGCTTATCGAATGGTCAAAATCTCTCACAAATCTTAAATGATTTACGTTTTTCAAACTTAGTCGTCACACAAGT
TGCTTTAGCAGATTACCATGGTGATACCCAGCAAACACTGACATTAATAATAGATAATTTAACAAAAATTCAGAAGGTTC
GAAAAAAATTGGTGGCTGTATCGACCTATCCCATCATCTTGTTAACTTTTCTAATCATCATCGTACTGGGATTAAAAACC
TATTTGCTACCCCAAGTTGAAACGGATAGTTTTGCGGGCACTGTGATCAATGCCTTACCTTTGATTTTTATCGGGTTGAG
CCTCGTGTTAACATTTTTTGCGTTAGGACTGATTCGGTATTTCAAATCAACATCACCATTGGCTAATGTCCAATGGTTAG
GTCGTCTTCCCTTGATCCGATCGTACTTAAAGCTTTATCTGACAGCGTTTTATGCGCGAGAGTGGGGAAACTTGGTTAAG
CAAGGTTTGGAGATGCGACAAATATTGGAGATGATGACCAGCCAACAGAATCTACTTTTTCGTGAAATTGGTCAAGATAT
AAGGCGGGCTATGCAAGTGGGGGAAGAATTTCACGACAAGATTAAGCAATACAGCTTTTTCACACCGGAGTTGGCCCTCA
TCATCGAATATGGTGAAATGAAGGGCAAATTAGGTGATGAATTACTCATCTATTCGGATGAATCCTGGACAAAATTTTTT
GAAAAGGTTGAAAGCGCCATGAATGTCATTCAGCCATTGGTTTTTTTGTTTGTCGCTTTGATGATTGTTTTAATCTATGC
AGCTATGTTGCTACCTATTTATGCTCAGATGGATCTGGGCATGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(47-164)

Type II secretion system (T2SS), protein F (T2SSF)

(232-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

50.725

95.568

0.485
  comYB Streptococcus mutans UA159

50.725

95.568

0.485
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

94.183

0.476
  comGB Lactococcus lactis subsp. cremoris KW2

49.267

94.46

0.465
  comGB/cglB Streptococcus pneumoniae R6

47.164

92.798

0.438
  comGB/cglB Streptococcus pneumoniae TIGR4

47.164

92.798

0.438
  comGB/cglB Streptococcus mitis NCTC 12261

47.164

92.798

0.438
  comGB/cglB Streptococcus pneumoniae Rx1

47.164

92.798

0.438
  comGB/cglB Streptococcus pneumoniae D39

47.164

92.798

0.438
  comGB/cglB Streptococcus mitis SK321

46.567

92.798

0.432

Multiple sequence alignment