Detailed information NTG0412301

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: H5022_RS12735
• Genome accession: NZ_CP060004
• Coordinates: 2679690..2680361 (+)
• Length: 223 a.a.
• NCBI ID: WP_003090494.1
• Uniprot ID: A0A0H2ZC88
• Organism: Pseudomonas aeruginosa strain WK172
• Function: repress competence development (predicted from homology) Competence regulation

Genomic Context

Location: 2674690..2685361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5022_RS12700 (H5022_12640)	ppyR	2675163..2675420 (+)	258	WP_003090502.1	psl/pyoverdine operon transcriptional regulator PpyR	-
H5022_RS12705 (H5022_12645)	-	2675407..2676600 (+)	1194	WP_003122598.1	NnrS family protein	-
H5022_RS12710 (H5022_12650)	-	2676601..2676867 (-)	267	WP_003115326.1	DUF5329 domain-containing protein	-
H5022_RS12715 (H5022_12655)	-	2676851..2677744 (-)	894	WP_003116712.1	hypothetical protein	-
H5022_RS12720 (H5022_12660)	-	2677771..2678853 (-)	1083	WP_003113385.1	hypothetical protein	-
H5022_RS12725 (H5022_12665)	-	2679068..2679376 (+)	309	WP_003090497.1	PepSY domain-containing protein	-
H5022_RS12730 (H5022_12670)	-	2679376..2679690 (+)	315	WP_003097704.1	PepSY domain-containing protein	-
H5022_RS12735 (H5022_12675)	ciaR	2679690..2680361 (+)	672	WP_003090494.1	two-component system response regulator CarR	Regulator
H5022_RS12740 (H5022_12680)	carS	2680358..2681695 (+)	1338	WP_003090493.1	two-component system sensor histidine kinase CarS	-
H5022_RS12745 (H5022_12685)	-	2681786..2682094 (+)	309	WP_003113384.1	hypothetical protein	-
H5022_RS12750 (H5022_12690)	-	2682099..2684243 (-)	2145	WP_003113383.1	methyl-accepting chemotaxis protein	-

Sequence

Protein

• Length: 223 a.a.
• Molecular weight: 24729.20 Da
• Isoelectric Point: 5.8445

>NTDB_id=412301 H5022_RS12735 WP_003090494.1 2679690..2680361(+) (ciaR) [Pseudomonas aeruginosa strain WK172]
MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA
RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR
YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide

• Length: 672 bp

>NTDB_id=412301 H5022_RS12735 WP_003090494.1 2679690..2680361(+) (ciaR) [Pseudomonas aeruginosa strain WK172]
ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGACGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT
GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGATCTGATCATCCTCGACCTCGGCC
TGCCCGGGCGTCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG
CGCGGTTCCTGGGCCGAACGCATCGATGGGCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA
GCTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC
GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC
TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA
TTCCAACGTCATCGAGGTCCACGTCAACCACCTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG
GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

• Response regulator receiver domain (Response_reg) (4-112)

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (147-216)

3D structure

• AlphaFold DB: A0A0H2ZC88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus mutans UA159	37.727	98.655	0.372
ciaR	Streptococcus pneumoniae Rx1	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae D39	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae R6	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae TIGR4	37.273	98.655	0.368
micA	Streptococcus pneumoniae Cp1015	35.683	100	0.363
vicR	Streptococcus mutans UA159	35.526	100	0.363