Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H5J27_RS30340 Genome accession   NZ_CP059995
Coordinates   6425346..6425969 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NY3045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6420346..6430969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS30320 (H5J27_30320) aroB 6420941..6422047 (-) 1107 WP_033977028.1 3-dehydroquinate synthase -
  H5J27_RS30325 (H5J27_30325) aroK 6422097..6422615 (-) 519 WP_003095833.1 shikimate kinase AroK -
  H5J27_RS30330 (H5J27_30330) pilQ 6422627..6424771 (-) 2145 WP_042170908.1 type 4a pilus secretin PilQ Machinery gene
  H5J27_RS30335 (H5J27_30335) comL/pilP 6424825..6425349 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  H5J27_RS30340 (H5J27_30340) pilO 6425346..6425969 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  H5J27_RS30345 (H5J27_30345) pilN 6425966..6426562 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  H5J27_RS30350 (H5J27_30350) pilM 6426562..6427626 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  H5J27_RS30355 (H5J27_30355) - 6427811..6430282 (+) 2472 WP_033977033.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=412242 H5J27_RS30340 WP_003103268.1 6425346..6425969(-) (pilO) [Pseudomonas aeruginosa strain NY3045]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=412242 H5J27_RS30340 WP_003103268.1 6425346..6425969(-) (pilO) [Pseudomonas aeruginosa strain NY3045]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGAGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401