Detailed information NTG0411696

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GRT08_RS12435
• Genome accession: NZ_CP047234
• Coordinates: 2551773..2552387 (-)
• Length: 204 a.a.
• NCBI ID: WP_071391595.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain FJ4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2546773..2557387

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GRT08_RS12405 (GRT08_12235)	rpsT	2547073..2547339 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GRT08_RS12410 (GRT08_12240)	holA	2547356..2548397 (-)	1042	Protein_2397	DNA polymerase III subunit delta	-
GRT08_RS12415 (GRT08_12245)	-	2548437..2548591 (-)	155	Protein_2398	hypothetical protein	-
GRT08_RS12420 (GRT08_12250)	-	2548632..2548766 (+)	135	WP_003152870.1	YqzM family protein	-
GRT08_RS12425 (GRT08_12255)	comEC	2548785..2551136 (-)	2352	WP_071391596.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GRT08_RS12430 (GRT08_12260)	-	2551137..2551706 (-)	570	WP_003152868.1	ComE operon protein 2	-
GRT08_RS12435 (GRT08_12265)	comEA	2551773..2552387 (-)	615	WP_071391595.1	helix-hairpin-helix domain-containing protein	Machinery gene
GRT08_RS12440 (GRT08_12270)	comER	2552445..2553266 (+)	822	WP_071391594.1	late competence protein ComER	-
GRT08_RS12445 (GRT08_12275)	-	2553335..2554072 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
GRT08_RS12450 (GRT08_12280)	rsfS	2554069..2554425 (-)	357	WP_003152864.1	ribosome silencing factor	-
GRT08_RS12455 (GRT08_12285)	yqeK	2554443..2555003 (-)	561	WP_031378934.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GRT08_RS12460 (GRT08_12290)	-	2554993..2555562 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GRT08_RS12465 (GRT08_12295)	yhbY	2555573..2555863 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GRT08_RS12470 (GRT08_12300)	aroE	2555857..2556699 (-)	843	WP_031378933.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22218.33 Da
• Isoelectric Point: 5.4841

>NTDB_id=411696 GRT08_RS12435 WP_071391595.1 2551773..2552387(-) (comEA) [Bacillus amyloliquefaciens strain FJ4]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTVSASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=411696 GRT08_RS12435 WP_071391595.1 2551773..2552387(-) (comEA) [Bacillus amyloliquefaciens strain FJ4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGTCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363