Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   GRT08_RS12425 Genome accession   NZ_CP047234
Coordinates   2548785..2551136 (-) Length   783 a.a.
NCBI ID   WP_071391596.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain FJ4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2543785..2556136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRT08_RS12390 (GRT08_12220) - 2544148..2544486 (-) 339 WP_007408270.1 YqxA family protein -
  GRT08_RS12395 (GRT08_12225) spoIIP 2544503..2545696 (-) 1194 WP_377802733.1 stage II sporulation protein P -
  GRT08_RS12400 (GRT08_12230) gpr 2545764..2546870 (-) 1107 WP_003152878.1 GPR endopeptidase -
  GRT08_RS12405 (GRT08_12235) rpsT 2547073..2547339 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  GRT08_RS12410 (GRT08_12240) holA 2547356..2548397 (-) 1042 Protein_2397 DNA polymerase III subunit delta -
  GRT08_RS12415 (GRT08_12245) - 2548437..2548591 (-) 155 Protein_2398 hypothetical protein -
  GRT08_RS12420 (GRT08_12250) - 2548632..2548766 (+) 135 WP_003152870.1 YqzM family protein -
  GRT08_RS12425 (GRT08_12255) comEC 2548785..2551136 (-) 2352 WP_071391596.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GRT08_RS12430 (GRT08_12260) - 2551137..2551706 (-) 570 WP_003152868.1 ComE operon protein 2 -
  GRT08_RS12435 (GRT08_12265) comEA 2551773..2552387 (-) 615 WP_071391595.1 helix-hairpin-helix domain-containing protein Machinery gene
  GRT08_RS12440 (GRT08_12270) comER 2552445..2553266 (+) 822 WP_071391594.1 late competence protein ComER -
  GRT08_RS12445 (GRT08_12275) - 2553335..2554072 (-) 738 WP_031378935.1 class I SAM-dependent methyltransferase -
  GRT08_RS12450 (GRT08_12280) rsfS 2554069..2554425 (-) 357 WP_003152864.1 ribosome silencing factor -
  GRT08_RS12455 (GRT08_12285) yqeK 2554443..2555003 (-) 561 WP_031378934.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GRT08_RS12460 (GRT08_12290) - 2554993..2555562 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  GRT08_RS12465 (GRT08_12295) yhbY 2555573..2555863 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86748.69 Da        Isoelectric Point: 9.0226

>NTDB_id=411694 GRT08_RS12425 WP_071391596.1 2548785..2551136(-) (comEC) [Bacillus amyloliquefaciens strain FJ4]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVIICLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRRIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFSVTSIQRCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAASSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPYRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQCQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=411694 GRT08_RS12425 WP_071391596.1 2548785..2551136(-) (comEC) [Bacillus amyloliquefaciens strain FJ4]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGGATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTATCATCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTATAAAATCCGGTCTCTTGAGGAAAAGAGACGCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCGGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCAATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCAGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTATATACGCTGCTGAGCGGTGCCGCCTCATCCGTATTGCGCGCATCTCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATTGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAACATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGCTTCCTTCTTCTTTTGCTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCATACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCTGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCCAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCGGGTGATCTTCAGTGTCAGGTGCTGTCTCCG
GAGTCATCTGACGGAAAGAGTAAAAATGATTCATCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(25-170)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Competence protein (Competence)

(208-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

56.088

98.595

0.553

Multiple sequence alignment