Detailed information NTG0411288

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D1605_RS06105
• Genome accession: NZ_CP047171
• Coordinates: 1354875..1356005 (+)
• Length: 376 a.a.
• NCBI ID: WP_118853444.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. fastidiosa strain IVIA5235
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1349875..1361005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D1605_RS06085 (D1605_006180)	-	1350162..1350986 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
D1605_RS06090 (D1605_006185)	-	1351620..1352447 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
D1605_RS06095 (D1605_006190)	-	1352805..1353017 (-)	213	WP_004087301.1	hypothetical protein	-
D1605_RS06100 (D1605_006195)	pilT	1353675..1354709 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
D1605_RS06105 (D1605_006200)	pilU	1354875..1356005 (+)	1131	WP_118853444.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D1605_RS06110 (D1605_006205)	-	1356698..1357666 (-)	969	WP_004087304.1	YafY family protein	-
D1605_RS06115 (D1605_006210)	-	1358515..1359324 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
D1605_RS06120 (D1605_006215)	hemC	1359378..1360295 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42410.62 Da
• Isoelectric Point: 6.4489

>NTDB_id=411288 D1605_RS06105 WP_118853444.1 1354875..1356005(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain IVIA5235]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLSQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=411288 D1605_RS06105 WP_118853444.1 1354875..1356005(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain IVIA5235]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTAAGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.308	97.606	0.598
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	50.562	94.681	0.479
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.436	96.277	0.399