Detailed information NTG0410652

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GE573_RS02440
• Genome accession: NZ_CP047119
• Coordinates: 469912..470526 (-)
• Length: 204 a.a.
• NCBI ID: WP_063094716.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain AK-0
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464912..475526

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GE573_RS02410 (GE573_00479)	rpsT	465215..465481 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GE573_RS02415 (GE573_00480)	holA	465498..466539 (-)	1042	Protein_485	DNA polymerase III subunit delta	-
GE573_RS02420	-	466579..466730 (-)	152	Protein_486	hypothetical protein	-
GE573_RS02425	-	466771..466905 (+)	135	WP_003152870.1	YqzM family protein	-
GE573_RS02430 (GE573_00481)	comEC	466924..469275 (-)	2352	WP_190664433.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GE573_RS02435 (GE573_00482)	-	469276..469845 (-)	570	WP_003152868.1	ComE operon protein 2	-
GE573_RS02440 (GE573_00483)	comEA	469912..470526 (-)	615	WP_063094716.1	helix-hairpin-helix domain-containing protein	Machinery gene
GE573_RS02445 (GE573_00484)	comER	470585..471406 (+)	822	WP_015240246.1	late competence protein ComER	-
GE573_RS02450 (GE573_00485)	-	471475..472212 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
GE573_RS02455 (GE573_00486)	rsfS	472209..472565 (-)	357	WP_007408260.1	ribosome silencing factor	-
GE573_RS02460 (GE573_00487)	yqeK	472583..473143 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GE573_RS02465 (GE573_00488)	-	473133..473702 (-)	570	WP_063094714.1	nicotinate-nucleotide adenylyltransferase	-
GE573_RS02470 (GE573_00489)	yhbY	473713..474003 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GE573_RS02475 (GE573_00490)	aroE	473997..474839 (-)	843	WP_061046606.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22094.19 Da
• Isoelectric Point: 5.5167

>NTDB_id=410652 GE573_RS02440 WP_063094716.1 469912..470526(-) (comEA) [Bacillus velezensis strain AK-0]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKEDTAGDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLGELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=410652 GE573_RS02440 WP_063094716.1 469912..470526(-) (comEA) [Bacillus velezensis strain AK-0]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAGAAGACACCGCAGGCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGGCGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.885	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363