Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   GP482_RS00940 Genome accession   NZ_CP046875
Coordinates   184094..185983 (+) Length   629 a.a.
NCBI ID   WP_158913248.1    Uniprot ID   A0AAE6UXY1
Organism   Streptococcus ruminicola strain CNU_77-61     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 179094..190983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GP482_RS00925 (GP482_00925) fmt 181072..182007 (+) 936 WP_074561288.1 methionyl-tRNA formyltransferase -
  GP482_RS00930 (GP482_00930) rsmB 181997..183319 (+) 1323 WP_158913246.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  GP482_RS00935 (GP482_00935) - 183357..184094 (+) 738 WP_074482688.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  GP482_RS00940 (GP482_00940) pknB 184094..185983 (+) 1890 WP_158913248.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  GP482_RS00945 (GP482_00945) liaF 186081..186776 (+) 696 WP_039695888.1 cell wall-active antibiotics response protein LiaF -
  GP482_RS00950 (GP482_00950) - 186773..187798 (+) 1026 WP_074565066.1 sensor histidine kinase -
  GP482_RS00955 (GP482_00955) - 187782..188426 (+) 645 WP_158913250.1 response regulator transcription factor -
  GP482_RS00960 (GP482_00960) - 188551..189951 (+) 1401 WP_158913252.1 Cof-type HAD-IIB family hydrolase -
  GP482_RS00965 (GP482_00965) - 189951..190307 (+) 357 WP_020916316.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 629 a.a.        Molecular weight: 68214.11 Da        Isoelectric Point: 6.6513

>NTDB_id=409097 GP482_RS00940 WP_158913248.1 184094..185983(+) (pknB) [Streptococcus ruminicola strain CNU_77-61]
MIQIGKLFAGRYRILKSIGRGGMADVYLAKDLILDNEEVAIKVLRTNYQTDQIAVARFQREARAMAELNHPNIVSIRDIG
EEDGQQFLVMEYVDGSDLKKYIQDHAPLSNNEVVRIMEEVLSAMTLAHQQGIVHRDLKPQNILLTKDGTVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIDENKNVP
QALENVVIKATAKRLSDRYASTFEMSRDLMTALSYNRSRERKLVFEDTENTKTLPKVTTSTPVPSTTEQLLKKQKAAKVN
REAADNKIAKAKTKKKKSHRMFGTLVKIFFAVVVVAIAIFTYLTLSTPASVNVPDVAGTSLADAKTSLTSAGLKVGDIHK
VASDTVQKGHVIKTDPTAGSSKKEGAKVDIYVSKGTSGFKMKDYTGQNYQEVIKELKDKHGISSDKIDVEWITGTNYDGD
TIISQTPGKGTKVASEDKITFKVAAIVMPNLIGYTYQDAIAELTALGLSSSHITVFQADATSSTGYSQVKTPSASAVVVA
QDPYFGAAIEGNVKLYFSAGSESQSSEVVPSQSVPESSETSETSESSSSSSSSSSTEESSQTDSSSVEQ

Nucleotide


Download         Length: 1890 bp        

>NTDB_id=409097 GP482_RS00940 WP_158913248.1 184094..185983(+) (pknB) [Streptococcus ruminicola strain CNU_77-61]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGGATCCTCAAATCTATCGGACGGGGTGGAATGGCAGATGTTTA
TCTTGCCAAAGATCTAATTCTTGATAATGAAGAAGTGGCTATCAAAGTCCTTCGAACTAATTATCAAACAGATCAAATTG
CCGTTGCTCGTTTCCAACGTGAAGCGCGTGCCATGGCAGAGCTAAACCATCCAAATATCGTTTCGATTCGTGATATTGGA
GAAGAAGACGGACAGCAATTCTTAGTAATGGAATATGTTGATGGTTCAGACTTGAAAAAATATATTCAAGACCATGCCCC
GCTTTCTAATAATGAAGTTGTTAGAATCATGGAAGAAGTCTTGTCTGCGATGACTTTGGCTCATCAACAAGGAATTGTTC
ACCGTGATTTGAAACCACAAAATATCTTGTTAACCAAAGACGGAACGGTTAAAGTTACCGACTTTGGTATCGCTGTAGCT
TTTGCAGAAACAAGTTTAACACAGACAAACTCAATGCTTGGTAGTGTACACTATTTATCACCTGAACAAGCGCGTGGTTC
AAAAGCCACTGTTCAAAGTGATATCTACGCTATGGGGATTATGTTGTTTGAAATGTTGACTGGTCACATCCCATATGATG
GTGATTCAGCTGTTACAATTGCTCTTCAACATTTCCAAAAACCACTTCCATCAATCATTGATGAAAATAAAAATGTTCCA
CAAGCTTTGGAAAATGTTGTCATTAAAGCAACTGCAAAACGTTTGAGTGATCGCTACGCCTCAACTTTTGAGATGAGCCG
TGATTTAATGACTGCTCTTTCATATAACCGCAGTCGTGAACGCAAACTTGTCTTTGAAGATACAGAAAATACGAAGACAC
TTCCAAAAGTAACAACTTCAACACCTGTTCCCTCAACTACGGAACAATTGTTGAAAAAACAAAAAGCGGCTAAAGTTAAT
CGTGAAGCTGCGGATAATAAAATTGCAAAAGCGAAAACGAAGAAGAAAAAATCACATCGTATGTTTGGTACTTTAGTGAA
AATTTTCTTTGCGGTCGTAGTAGTTGCAATTGCTATCTTTACTTACTTGACGCTAAGCACACCAGCTTCAGTCAACGTTC
CAGATGTAGCAGGAACAAGTTTAGCAGATGCTAAGACAAGTTTAACTTCAGCTGGACTTAAAGTTGGTGATATTCATAAA
GTAGCTAGTGATACTGTGCAAAAAGGTCATGTTATTAAGACTGACCCAACAGCAGGTTCATCTAAAAAAGAAGGTGCCAA
AGTTGACATTTATGTGTCAAAAGGAACATCAGGCTTTAAGATGAAAGATTATACTGGTCAAAACTATCAAGAAGTTATCA
AAGAACTCAAAGATAAGCACGGTATTTCAAGTGATAAGATTGATGTTGAATGGATAACAGGTACTAACTATGACGGTGAT
ACTATCATTAGTCAAACACCTGGAAAAGGTACAAAAGTAGCTAGTGAAGATAAGATTACTTTCAAGGTTGCAGCAATCGT
TATGCCAAACCTTATTGGTTATACTTATCAAGATGCAATTGCTGAATTGACTGCACTTGGCTTATCAAGCTCACATATTA
CAGTATTCCAAGCAGATGCTACTTCATCAACTGGATACTCTCAAGTCAAAACACCATCTGCCTCTGCAGTTGTTGTGGCA
CAAGATCCTTACTTTGGCGCAGCTATTGAAGGAAATGTGAAACTTTACTTCTCAGCTGGAAGTGAATCTCAGTCTTCAGA
AGTCGTACCATCTCAATCTGTTCCTGAGTCATCTGAGACAAGTGAAACTAGTGAGTCAAGTTCTTCAAGTAGTAGCAGTA
GCAGCACTGAAGAAAGTTCACAAACCGACTCATCATCAGTTGAACAATAA

Domains

Predicted by InterproScan.

PASTA domain (PASTA)

(440-503)

PASTA domain (PASTA)

(507-578)

PASTA domain (PASTA)

(371-434)

Protein kinase domain (Pkinase)

(12-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.849

90.779

0.598
  stkP/pknB Streptococcus salivarius strain HSISS4

62.669

94.118

0.59
  stkP Streptococcus pneumoniae TIGR4

58.569

84.42

0.494
  stkP Streptococcus pneumoniae D39

58.38

84.42

0.493
  stkP Streptococcus pneumoniae R6

58.38

84.42

0.493
  stkP Streptococcus pneumoniae Rx1

58.192

84.42

0.491

Multiple sequence alignment