Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GO174_RS11845 Genome accession   NZ_CP046857
Coordinates   2502088..2502678 (-) Length   196 a.a.
NCBI ID   WP_158125469.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0233     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2497088..2507678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO174_RS11825 aroB 2498006..2499091 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  GO174_RS11830 aroK 2499119..2499637 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GO174_RS11835 pilQ 2499825..2501561 (-) 1737 WP_233921186.1 type IV pilus secretin PilQ family protein Machinery gene
  GO174_RS11840 pilP 2501586..2502098 (-) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  GO174_RS11845 pilO 2502088..2502678 (-) 591 WP_158125469.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO174_RS11850 pilN 2502671..2503261 (-) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GO174_RS11855 pilM 2503251..2504255 (-) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene
  GO174_RS11860 - 2504398..2506878 (+) 2481 WP_158125471.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22751.15 Da        Isoelectric Point: 4.7023

>NTDB_id=409005 GO174_RS11845 WP_158125469.1 2502088..2502678(-) (pilO) [Vibrio fluvialis strain 2015AW-0233]
MANLQELELDEIVEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGERQNQEFLYRLPLNIELTGNYHSIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=409005 GO174_RS11845 WP_158125469.1 2502088..2502678(-) (pilO) [Vibrio fluvialis strain 2015AW-0233]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGTTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCGCTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GTTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAGGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAGCATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

80.102

100

0.801
  pilO Vibrio campbellii strain DS40M4

67.895

96.939

0.658

Multiple sequence alignment