Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GO174_RS11835 Genome accession   NZ_CP046857
Coordinates   2499825..2501561 (-) Length   578 a.a.
NCBI ID   WP_233921186.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0233     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494825..2506561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO174_RS11815 - 2495527..2496357 (-) 831 WP_020329450.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GO174_RS11820 - 2496455..2497960 (-) 1506 WP_158125468.1 AAA family ATPase -
  GO174_RS11825 aroB 2498006..2499091 (-) 1086 WP_024374091.1 3-dehydroquinate synthase -
  GO174_RS11830 aroK 2499119..2499637 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GO174_RS11835 pilQ 2499825..2501561 (-) 1737 WP_233921186.1 type IV pilus secretin PilQ family protein Machinery gene
  GO174_RS11840 pilP 2501586..2502098 (-) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  GO174_RS11845 pilO 2502088..2502678 (-) 591 WP_158125469.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO174_RS11850 pilN 2502671..2503261 (-) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GO174_RS11855 pilM 2503251..2504255 (-) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62410.35 Da        Isoelectric Point: 4.5373

>NTDB_id=409003 GO174_RS11835 WP_233921186.1 2499825..2501561(-) (pilQ) [Vibrio fluvialis strain 2015AW-0233]
MIKGLNASTAWGIRCLFVGACLLLSAATLAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLNIELLKT
DVSDDKLYLLDVRDFATAVENIEVFRQQPSTLLVATVQGEFNYDYSLKGNYLEVVIRKLKAEEKAKPKSVLEKEGKLISI
NFQDIPVRNVLQLIADYNEFNLVVSDSVRGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KARMAEELGDLQSDILIINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLIRELPENIAVIRDIVASLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGGNNSGGSGDGVAIDDFLNVNLAATSANASSIA
FQVAKLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPVLGALFRRSYE
QMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=409003 GO174_RS11835 WP_233921186.1 2499825..2501561(-) (pilQ) [Vibrio fluvialis strain 2015AW-0233]
ATGATTAAAGGACTAAATGCCTCCACGGCGTGGGGAATACGTTGTCTGTTTGTGGGCGCATGCCTGCTGTTATCTGCCGC
CACACTTGCTCAGGAGGCGGCGGCCAATCAGCTGAAGAATATCGATTTTCGCATCAACAAAGACAAAGCGGCGGTACTGA
TTGTCGAACTGGCTTCCGCCTCGGCCGTGGTGGATGTGCAGAAAACCGAGCAAGGCCTGAATATCGAACTGCTGAAAACC
GATGTCAGCGACGACAAACTCTACCTGCTCGATGTGCGCGACTTTGCCACCGCCGTGGAAAACATCGAAGTGTTCCGCCA
GCAGCCCAGCACCCTGTTGGTCGCAACGGTGCAGGGGGAGTTCAATTATGATTACAGCCTGAAGGGTAACTATCTGGAAG
TGGTGATCCGCAAGCTAAAAGCCGAGGAGAAAGCCAAACCCAAAAGCGTACTAGAAAAAGAAGGCAAGCTGATTTCGATT
AACTTTCAGGATATTCCGGTGCGCAATGTGCTGCAGTTGATCGCAGACTACAATGAGTTCAATCTGGTGGTTTCCGATTC
AGTGCGCGGCAACCTGACGCTGCGCCTTGATGGCGTGCCCTGGCAGCAGGTGCTGGACATTATTCTGCAAGTCAAAGGTC
TGGATAAGCGCGTCGACGGCAATGTGATTCTGGTGGCGCCCAAAGAAGAGCTGGATCTGCGTGAAAAGCAGCAGTTGGAA
AAAGCCCGCATGGCGGAAGAACTCGGCGATCTGCAATCGGATATTCTCATCATCAACTTTGCCAAAGCGTCTGACATTGC
GCAGATGATTGGCGGCGATGGGGCGGTCAATATGTTGTCGGAGCGTGGCTCGATCAGCATTGATGAACGCACCAATGCGC
TACTGATTCGTGAACTGCCGGAGAACATTGCTGTGATTCGCGATATCGTGGCGTCGCTCGATATTCCGGTCAAGCAAGTA
CAGATTGAAGCGCGTATTGTGACGGTCAATGAAGGTAACTTGGATGAACTGGGTGTGCGCTGGGGTTTCAGCTCCACCAA
TGGCAGCACGACCACGGGTGGCTCGATTGAAAGCAATCTGGGGGCAATTGGTTTGTACGATGGCGGTAATAATAGTGGCG
GTTCTGGTGATGGCGTTGCGATTGATGATTTCCTCAACGTTAACCTCGCAGCCACCTCAGCCAATGCGTCCAGCATTGCG
TTTCAGGTCGCCAAGCTTGGCGCAGATACCTTGCTCGATCTTGAACTGTCAGCCATGCAACGCGAGTCAAAAGCGGAAAT
CATCTCCAGTCCCAGACTGATCACGACCAACAAGCAGCCGGCGTATATTGAGCAGGGTACTGAGATTCCGTACCTGGAAT
CCTCATCCAGTGGCGCCACCAGTGTGGCATTCAAGAAGGCAGTACTCAGCCTCAAAGTGACGCCACAAATCACCCCCGAT
AACCGTCTGGTGCTGGATTTGAGCGTCACTCAGGATCGGCCGGGTGAAGTGGTGAAAACCGGTACTGGCGAGGCGGTGGC
GATCAACACGCAGCGCATTGGTACACAGGTGCTGGTCAACAACGGTGAGACCGTGGTTCTGGGCGGCATTTTCCAGCACA
GTATTACGAATTCAGTCGACAAGGTGCCGCTGCTGGGGGACTTGCCCGTGTTGGGCGCGCTGTTCCGTCGCAGCTACGAA
CAAATGGGCAAAAGTGAGCTGCTGATCTTCGTAACACCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(418-576)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-319)

Secretin and TonB N terminus short domain (STN)

(180-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

81.693

100

0.818
  pilQ Vibrio cholerae strain A1552

81.693

100

0.818
  pilQ Vibrio campbellii strain DS40M4

79.235

94.983

0.753
  pilQ Legionella pneumophila strain Lp02

42.364

95.156

0.403
  pilQ Legionella pneumophila strain ERS1305867

42.364

95.156

0.403
  pilQ Pseudomonas aeruginosa PAK

40.721

96.021

0.391
  comQ Acinetobacter baylyi ADP1

37.973

100

0.382

Multiple sequence alignment