Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GO174_RS11855 Genome accession   NZ_CP046857
Coordinates   2503251..2504255 (-) Length   334 a.a.
NCBI ID   WP_158125470.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 2015AW-0233     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2498251..2509255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO174_RS11830 aroK 2499119..2499637 (-) 519 WP_004728569.1 shikimate kinase AroK -
  GO174_RS11835 pilQ 2499825..2501561 (-) 1737 WP_233921186.1 type IV pilus secretin PilQ family protein Machinery gene
  GO174_RS11840 pilP 2501586..2502098 (-) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  GO174_RS11845 pilO 2502088..2502678 (-) 591 WP_158125469.1 type 4a pilus biogenesis protein PilO Machinery gene
  GO174_RS11850 pilN 2502671..2503261 (-) 591 WP_100267596.1 PilN domain-containing protein Machinery gene
  GO174_RS11855 pilM 2503251..2504255 (-) 1005 WP_158125470.1 type IV pilus assembly protein PilM Machinery gene
  GO174_RS11860 - 2504398..2506878 (+) 2481 WP_158125471.1 PBP1A family penicillin-binding protein -
  GO174_RS11865 oxyR 2506939..2507835 (-) 897 WP_020329438.1 DNA-binding transcriptional regulator OxyR -
  GO174_RS11870 - 2507979..2508713 (+) 735 WP_020329437.1 glutathione peroxidase -
  GO174_RS11875 - 2508842..2509105 (+) 264 WP_158125472.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36871.18 Da        Isoelectric Point: 6.6593

>NTDB_id=409007 GO174_RS11855 WP_158125470.1 2503251..2504255(-) (pilM) [Vibrio fluvialis strain 2015AW-0233]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSEDIFADNHTLNYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSELDASEQEFAVYQAFSHQSPLPIEELSLDFVVTGEKTLGRSSSLTYQVYATRREVIDNRLSAMSKAGFEP
LLFDMQIHGLLNIWQWVSRAQQRTDWMLVDVGFSQTSLCIDFADKTPFYKDIPLGTRQLVAATSGGNADLTSPQAHFIQD
LVERLARQIQLFLSLQGHQSLAGIWLSGGGAATEGLAQAISERLVLPCELFNPLAEFTCRASLRHHPQVDLQRFSTAAGL
ALRGLNWLENGHAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=409007 GO174_RS11855 WP_158125470.1 2503251..2504255(-) (pilM) [Vibrio fluvialis strain 2015AW-0233]
ATGGGTAAATCATTAGTTACAGGTGTCGATATTGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAAGGCGAATC
GTATTCGCTGGTCGGTTACCAAGAGATAGTCGTATCCGAGGATATTTTCGCTGATAACCACACGCTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGTAAAGTGGCGCTGGCTGTACCGGACAACGCA
GTAATAAGTAAGGTATTACAGATAGATAGCGAACTGGATGCCAGCGAACAAGAGTTCGCGGTTTATCAGGCTTTTTCCCA
TCAATCGCCGCTACCCATCGAAGAGTTGAGCCTGGATTTCGTTGTCACCGGAGAAAAAACACTGGGGCGCAGCAGTTCGT
TGACGTATCAGGTCTATGCAACCCGACGTGAAGTGATCGACAACCGTTTGTCTGCGATGAGCAAAGCGGGTTTTGAGCCG
CTGCTGTTTGATATGCAGATTCACGGCCTGCTCAATATCTGGCAATGGGTCAGCCGTGCTCAGCAACGCACCGATTGGAT
GCTGGTTGATGTCGGGTTCAGTCAAACCTCATTGTGCATCGACTTTGCCGATAAAACGCCGTTTTACAAAGACATTCCGT
TGGGAACCCGCCAACTGGTTGCGGCGACCTCCGGTGGTAACGCTGACCTTACCAGCCCGCAAGCACACTTCATTCAGGAT
TTGGTTGAGCGTCTGGCGCGCCAGATCCAGCTGTTTCTTTCTCTGCAAGGCCATCAGTCGCTGGCCGGTATCTGGCTATC
CGGTGGCGGCGCGGCAACGGAAGGTTTGGCGCAGGCGATTAGCGAACGTCTGGTTCTGCCTTGCGAGCTGTTTAACCCTC
TGGCGGAGTTTACTTGCCGCGCATCGCTACGCCACCATCCTCAGGTTGATTTGCAGCGTTTCAGCACTGCGGCAGGCTTG
GCGCTGCGCGGGCTCAATTGGCTGGAGAACGGCCATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

65.68

100

0.665
  pilM Vibrio campbellii strain DS40M4

47.826

100

0.494

Multiple sequence alignment