Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GPY15_RS10765 Genome accession   NZ_CP046810
Coordinates   2368170..2369039 (+) Length   289 a.a.
NCBI ID   WP_158161908.1    Uniprot ID   -
Organism   Grimontia hollisae strain 1078-81     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2363170..2374039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY15_RS10745 nadC 2363566..2364471 (+) 906 WP_158161900.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY15_RS10750 pilA 2364699..2365133 (+) 435 WP_158161902.1 pilin Machinery gene
  GPY15_RS10755 pilB 2365133..2366827 (+) 1695 WP_158161904.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY15_RS10760 pilC 2366839..2368062 (+) 1224 WP_158161906.1 type II secretion system F family protein Machinery gene
  GPY15_RS10765 pilD 2368170..2369039 (+) 870 WP_158161908.1 prepilin peptidase Machinery gene
  GPY15_RS10770 coaE 2369043..2369666 (+) 624 WP_005502108.1 dephospho-CoA kinase -
  GPY15_RS10775 zapD 2369656..2370396 (+) 741 WP_005502110.1 cell division protein ZapD -
  GPY15_RS10780 yacG 2370431..2370628 (+) 198 WP_115660105.1 DNA gyrase inhibitor YacG -
  GPY15_RS10785 mutT 2370713..2371117 (-) 405 WP_114996183.1 8-oxo-dGTP diphosphatase MutT -
  GPY15_RS10790 secA 2371206..2373932 (-) 2727 WP_158162349.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31648.60 Da        Isoelectric Point: 6.2150

>NTDB_id=408190 GPY15_RS10765 WP_158161908.1 2368170..2369039(+) (pilD) [Grimontia hollisae strain 1078-81]
MDWLFDSPGLYVFLASLFGLIVGSFLNVVIYRLPKMMESQWRQECHASFPQEMPAPSESEVFNLSLPRSHCPSCHAPVRA
IDNIPVLSWLILGGKCRSCKTNISVRYPLIEILTAVLSGVVAYTLSGYWSLAVIGATFVLVVLCFIDLDTLLLPDQMTLP
LMWAGILLALTGLSPVSLQDAVIGAMAGYLSLWSVFQLFKLLTGKEGMGYGDFKLLAALGAWLGWQALPMVILMASMAGA
IGGIITMRVKGTGKETPFSFGPYLAIAGWISLLWGNMILDWYLTSYLGY

Nucleotide


Download         Length: 870 bp        

>NTDB_id=408190 GPY15_RS10765 WP_158161908.1 2368170..2369039(+) (pilD) [Grimontia hollisae strain 1078-81]
ATGGATTGGCTTTTCGACTCCCCGGGTCTTTACGTTTTCCTTGCCTCACTGTTCGGCCTGATTGTAGGCAGCTTCCTCAA
TGTGGTTATCTATCGTTTACCCAAAATGATGGAGAGCCAGTGGCGACAGGAATGCCACGCCAGTTTTCCGCAAGAGATGC
CTGCGCCCAGTGAAAGTGAGGTATTCAACCTCAGCCTGCCGCGTTCTCACTGTCCGTCATGCCATGCGCCCGTCCGCGCG
ATTGACAACATTCCGGTATTAAGCTGGCTGATCCTTGGCGGCAAGTGCCGTTCCTGCAAAACCAACATCAGCGTCCGCTA
TCCCTTGATTGAAATTCTGACTGCCGTGCTGTCAGGTGTTGTGGCCTATACGCTGTCAGGGTATTGGTCACTCGCCGTGA
TTGGTGCCACTTTCGTGCTGGTTGTGCTCTGTTTTATCGACCTTGATACCCTGTTGCTGCCAGACCAGATGACACTGCCC
CTGATGTGGGCCGGTATTTTACTTGCGCTTACCGGTCTCTCCCCGGTTTCACTCCAGGATGCGGTGATCGGCGCGATGGC
GGGATACCTGTCACTGTGGAGCGTGTTCCAGCTGTTTAAGCTGCTGACGGGCAAGGAAGGGATGGGTTATGGCGATTTCA
AGCTCCTTGCAGCCCTTGGGGCCTGGCTGGGTTGGCAGGCATTGCCTATGGTTATTCTGATGGCGTCTATGGCTGGTGCG
ATTGGTGGCATCATCACAATGCGCGTGAAAGGCACAGGGAAAGAAACACCTTTTTCATTTGGCCCTTACCTGGCCATTGC
GGGCTGGATAAGTTTGCTGTGGGGCAATATGATCCTGGACTGGTATTTAACATCTTATCTGGGTTATTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(138-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

62.284

100

0.623
  pilD Vibrio campbellii strain DS40M4

63.83

97.578

0.623
  pilD Neisseria gonorrhoeae MS11

50.903

95.848

0.488
  pilD Acinetobacter nosocomialis M2

49.462

96.54

0.478
  pilD Acinetobacter baumannii D1279779

49.462

96.54

0.478

Multiple sequence alignment