Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY45_RS08260 Genome accession   NZ_CP046785
Coordinates   1611466..1611939 (-) Length   157 a.a.
NCBI ID   WP_005462544.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2013V-1136     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1606466..1616939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY45_RS08240 coaE 1606977..1607591 (-) 615 WP_069494409.1 dephospho-CoA kinase -
  GPY45_RS08245 pilD 1607592..1608461 (-) 870 WP_089203077.1 A24 family peptidase Machinery gene
  GPY45_RS08250 pilC 1608526..1609749 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  GPY45_RS08255 pilB 1609775..1611460 (-) 1686 WP_089203076.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY45_RS08260 pilA 1611466..1611939 (-) 474 WP_005462544.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY45_RS08265 nadC 1612202..1613089 (-) 888 WP_005479699.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY45_RS08270 ampD 1613182..1613733 (+) 552 WP_025570907.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY45_RS08275 pdhR 1614139..1614906 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16598.20 Da        Isoelectric Point: 8.4679

>NTDB_id=407746 GPY45_RS08260 WP_005462544.1 1611466..1611939(-) (pilA) [Vibrio parahaemolyticus strain 2013V-1136]
MKHSKQKKQQGFTLIELMIVVAIIGILSAFAVPAYQNYTKKATLSEFPKAAAAAKLAVELCAHENASNETTFISECVKEE
NGIPAKFVLNNIEISVVKATDASVSGAIAVIAAADKVKGPIAKGEQYIMAASYKTQGLEWGASCKAGGKAQTDYCPN

Nucleotide


Download         Length: 474 bp        

>NTDB_id=407746 GPY45_RS08260 WP_005462544.1 1611466..1611939(-) (pilA) [Vibrio parahaemolyticus strain 2013V-1136]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACGCTAATTGAATTGATGATTGTGGTGGCGATTATCGGCATCTT
ATCTGCTTTCGCAGTTCCTGCTTACCAAAATTATACTAAGAAAGCGACATTGTCAGAATTTCCAAAAGCAGCTGCGGCAG
CCAAATTAGCAGTAGAATTATGTGCCCATGAGAATGCCTCAAATGAAACAACATTTATTAGTGAATGTGTAAAGGAAGAA
AATGGAATTCCCGCTAAATTCGTTTTGAATAATATTGAAATTAGCGTTGTTAAAGCCACAGATGCTAGCGTTTCTGGTGC
GATAGCAGTTATTGCCGCAGCTGATAAAGTTAAGGGACCTATTGCTAAAGGCGAACAATATATCATGGCTGCATCATATA
AAACTCAAGGCTTAGAGTGGGGGGCTTCATGTAAAGCTGGCGGTAAAGCACAAACAGACTACTGCCCAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

57.325

100

0.573
  pilA Haemophilus influenzae Rd KW20

43.056

91.72

0.395
  pilA Haemophilus influenzae 86-028NP

41.781

92.994

0.389

Multiple sequence alignment