Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSEFU_RS02135 Genome accession   NC_015556
Coordinates   473572..475782 (+) Length   736 a.a.
NCBI ID   WP_013789551.1    Uniprot ID   F6AGT0
Organism   Pseudomonas fulva 12-X     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468572..480782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEFU_RS02115 (Psefu_0422) pilM 470746..471810 (+) 1065 WP_013789547.1 pilus assembly protein PilM Machinery gene
  PSEFU_RS02120 (Psefu_0423) pilN 471810..472382 (+) 573 WP_013789548.1 type 4a pilus biogenesis protein PilN -
  PSEFU_RS02125 (Psefu_0424) pilO 472379..473002 (+) 624 WP_013789549.1 type 4a pilus biogenesis protein PilO -
  PSEFU_RS02130 (Psefu_0425) pilP 472999..473517 (+) 519 WP_013789550.1 type 4a pilus biogenesis lipoprotein PilP -
  PSEFU_RS02135 (Psefu_0426) pilQ 473572..475782 (+) 2211 WP_013789551.1 type IV pilus secretin PilQ Machinery gene
  PSEFU_RS02140 (Psefu_0427) aroK 475786..476304 (+) 519 WP_041705688.1 shikimate kinase AroK -
  PSEFU_RS02145 (Psefu_0428) aroB 476356..477462 (+) 1107 WP_013789553.1 3-dehydroquinate synthase -
  PSEFU_RS02150 (Psefu_0429) - 477470..479086 (+) 1617 WP_013789554.1 AAA family ATPase -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 78039.28 Da        Isoelectric Point: 4.9155

>NTDB_id=40760 PSEFU_RS02135 WP_013789551.1 473572..475782(+) (pilQ) [Pseudomonas fulva 12-X]
MNSSLSRLSISLLVAMLSPAVLAANLQALDVAALPGDRVELKLSFDEPVAAPRGYTIEQPARIALDLPGVSNKLGVKNRE
LGMGNARSVTVVEAKDRTRLIINLTNLSPYNTRTEGNNLFVQVGTGVATSATTTAAAPVTAPAAAAARPAQAATPVSAPT
APTAPKVFGAVNKSISNIDFQRGEQGEGNIVINLSDASVSPDIQERGGKIILNFNRTQLPEALRVRLDVKDFATPVQFVS
ATGKGDDASIVIEPVGAFDYLAYQTENKLTLSVKPLTQEDAEKRQADRFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNL
VASDTVAGNITLRLQNVPWDQALDLVLKTKGLDKRQVGNVLLVAPAEEIAAREKQELESQKQIAELAPLRRELIQVNYAK
AADMAKLFQSVTSANGGVADDRGSITVDERTNSIIAYQTQERLDELRRIVAQLDVAVRQVMIEARIVEANVNYSKALGVR
WGGAIGAGSRYTAYGKNGNVGIRDATGGTTSSGCGPFAGNCTLPTTAAAGGGGSSGSSPTPFVDMGVANSTSGIGIGFIT
NSAIIDLQLSAMESSGNGEIVSQPKVVTSDKETAKILKGQEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEV
KVNKDAPDYQNELNGVPPISKNEVNAKVLVADGETIVIGGVFENTQTKSTEKVPFLGDVPYLGRLFRRDTVNDNKTELLV
FLTPRIMNTSAVAVSQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=40760 PSEFU_RS02135 WP_013789551.1 473572..475782(+) (pilQ) [Pseudomonas fulva 12-X]
ATGAACAGCTCCCTCTCTCGCCTCAGTATTTCGCTGCTGGTGGCTATGCTCTCTCCTGCCGTTCTGGCCGCCAATCTGCA
GGCGCTGGATGTGGCTGCTCTGCCGGGTGACCGTGTCGAGTTGAAACTGTCTTTCGACGAGCCGGTGGCGGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGTATCGCTCTGGATCTGCCGGGCGTATCCAACAAGCTGGGCGTGAAGAATCGCGAA
CTGGGCATGGGTAACGCGCGCAGTGTCACCGTGGTCGAGGCCAAGGACCGTACGCGGCTGATCATCAACCTGACCAATCT
GTCGCCTTACAACACTCGCACCGAAGGCAACAACCTGTTCGTACAGGTCGGTACCGGCGTGGCCACTTCGGCGACCACGA
CCGCCGCAGCGCCCGTGACTGCGCCTGCCGCAGCGGCCGCCCGTCCTGCTCAGGCTGCAACGCCAGTTTCCGCGCCGACC
GCTCCGACCGCTCCGAAAGTATTCGGCGCCGTGAACAAGTCGATCAGCAATATCGACTTCCAGCGCGGCGAGCAGGGTGA
GGGCAATATCGTCATCAACCTCTCCGATGCCTCCGTCAGCCCCGATATTCAGGAGCGTGGTGGCAAGATCATCCTCAACT
TCAATCGCACCCAACTGCCTGAGGCTCTGCGGGTGCGTCTGGACGTCAAGGACTTCGCCACGCCCGTGCAGTTCGTCAGT
GCCACTGGTAAAGGCGACGATGCCAGCATCGTTATCGAGCCGGTGGGTGCCTTCGATTACCTGGCCTACCAGACCGAAAA
CAAGCTGACCCTCAGCGTCAAGCCGCTGACCCAGGAAGATGCAGAGAAGCGTCAGGCTGACCGCTTCGCCTATACCGGCG
AGAAGCTGTCGCTGAACTTCCAGGACATCGACGTACGCTCGGTGCTGCAGCTGATCGCCGACTTCACCGATCTGAACCTG
GTCGCCAGTGATACGGTTGCGGGCAATATCACCTTGCGTCTGCAGAATGTGCCGTGGGATCAGGCGCTGGATTTGGTGCT
GAAAACCAAGGGGCTGGACAAGCGTCAGGTCGGCAACGTATTGCTGGTCGCACCAGCGGAGGAAATCGCTGCCCGCGAGA
AACAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGCTGCGTCGTGAGCTGATCCAGGTCAACTACGCCAAG
GCTGCCGATATGGCCAAGCTGTTCCAGTCGGTGACCAGTGCGAATGGTGGTGTCGCCGATGATCGTGGTTCGATTACTGT
CGATGAGCGTACCAATAGCATCATTGCTTACCAAACTCAGGAGCGCCTGGACGAACTGCGTCGTATTGTTGCTCAGCTAG
ACGTAGCGGTACGTCAAGTGATGATTGAGGCGCGGATTGTTGAGGCGAACGTCAACTACAGCAAGGCGCTGGGTGTTCGT
TGGGGTGGGGCAATAGGTGCCGGCTCGCGTTACACCGCGTACGGAAAAAATGGCAACGTGGGTATTAGGGATGCGACCGG
TGGTACTACCAGTAGTGGCTGTGGGCCGTTCGCCGGGAACTGCACATTGCCCACGACTGCCGCTGCTGGCGGCGGCGGTA
GCTCGGGTAGCTCGCCAACACCGTTTGTCGACATGGGTGTGGCGAATAGTACCTCTGGGATCGGTATAGGTTTCATTACC
AACAGCGCGATTATCGACCTCCAGCTTTCTGCCATGGAAAGTTCTGGCAACGGTGAAATCGTTTCGCAGCCTAAGGTTGT
GACCTCTGATAAAGAGACCGCGAAAATTCTCAAGGGTCAGGAAATCCCTTATCAAGAGGCCAGCTCCAGCGGTGCGACAA
GTACTTCATTCAAGGAGGCTGCGTTGTCTCTTGAGGTAACCCCGCAAATTACTCCTGATAACCGCATTATCATGGAGGTG
AAGGTCAACAAGGATGCGCCTGATTATCAGAACGAATTGAATGGTGTTCCGCCTATCAGTAAGAACGAGGTAAATGCCAA
GGTCCTGGTAGCTGATGGCGAGACTATCGTCATTGGGGGGGTATTTGAGAATACGCAGACAAAGTCCACCGAAAAGGTAC
CGTTCCTTGGTGATGTGCCTTATCTCGGCCGCCTGTTCCGGCGCGATACCGTCAACGACAACAAGACCGAGTTGCTGGTT
TTCCTCACTCCGCGCATCATGAACACTTCCGCGGTGGCTGTGAGCCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(393-459)

Bacterial type II and III secretion system protein (Secretin)

(571-727)

Secretin and TonB N terminus short domain (STN)

(319-365)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6AGT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.797

100

0.75
  pilQ Acinetobacter baumannii D1279779

37.873

100

0.397
  pilQ Acinetobacter baumannii strain A118

37.873

100

0.397
  comQ Acinetobacter baylyi ADP1

39.917

98.37

0.393

Multiple sequence alignment