Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PSEFU_RS02115 Genome accession   NC_015556
Coordinates   470746..471810 (+) Length   354 a.a.
NCBI ID   WP_013789547.1    Uniprot ID   F6AGS6
Organism   Pseudomonas fulva 12-X     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465746..476810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEFU_RS02105 (Psefu_0420) - 466751..468019 (+) 1269 WP_013789545.1 malic enzyme-like NAD(P)-binding protein -
  PSEFU_RS02110 (Psefu_0421) - 468126..470564 (-) 2439 WP_041705686.1 penicillin-binding protein 1A -
  PSEFU_RS02115 (Psefu_0422) pilM 470746..471810 (+) 1065 WP_013789547.1 pilus assembly protein PilM Machinery gene
  PSEFU_RS02120 (Psefu_0423) pilN 471810..472382 (+) 573 WP_013789548.1 type 4a pilus biogenesis protein PilN -
  PSEFU_RS02125 (Psefu_0424) pilO 472379..473002 (+) 624 WP_013789549.1 type 4a pilus biogenesis protein PilO -
  PSEFU_RS02130 (Psefu_0425) pilP 472999..473517 (+) 519 WP_013789550.1 type 4a pilus biogenesis lipoprotein PilP -
  PSEFU_RS02135 (Psefu_0426) pilQ 473572..475782 (+) 2211 WP_013789551.1 type IV pilus secretin PilQ Machinery gene
  PSEFU_RS02140 (Psefu_0427) aroK 475786..476304 (+) 519 WP_041705688.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38007.34 Da        Isoelectric Point: 4.4260

>NTDB_id=40759 PSEFU_RS02115 WP_013789547.1 470746..471810(+) (pilM) [Pseudomonas fulva 12-X]
MLGLFSKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVRSVSV
AVAGSAVITKTVEMEAGLSDDELENQLKLEADQYIPYPLEEVAIDFEVQGISPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYELLVEQLGGAHDDLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
DEAGLAKKQGGLPDDYESEVLQPFKDALVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=40759 PSEFU_RS02115 WP_013789547.1 470746..471810(+) (pilM) [Pseudomonas fulva 12-X]
GTGCTAGGGCTCTTCAGTAAAAAAGCGAATACGCTTTTGGGGATCGATATAAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGTAGCCGCTACAAGGTAGAGGCCTACGCTGTCGAGCCGCTCCCCGCCAATGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGTGTCGGCCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTGCGCTCGGTTTCCGTT
GCCGTCGCCGGCTCCGCGGTGATCACCAAGACCGTGGAGATGGAAGCAGGCCTGTCGGACGACGAACTGGAGAATCAGCT
CAAGCTCGAAGCCGATCAGTACATTCCCTACCCCCTTGAAGAGGTAGCGATCGACTTCGAAGTTCAGGGTATTTCGCCGC
GTAATCCCGAGCGGGTCGAAGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTTCGCGAGGCTGCCCTGGCGCTC
GCCGGCCTGACCGCCAAGGTCGTCGATGTCGAAGCATATGCCCTCGAGCGTGCCTATGAGCTGCTTGTCGAGCAGTTGGG
CGGCGCCCACGACGATCTGACCGTAGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCACCATCTATACCCGCGAACAACTGTTCGGCGGCCGCCAACTGACCGAAGAGATCCAGCGTCGCTACGGTCTTTCCGTC
GATGAAGCCGGGCTGGCCAAGAAGCAGGGCGGCCTGCCTGACGATTATGAGAGCGAAGTGTTACAGCCGTTCAAGGACGC
GCTGGTGCAGCAGGTGTCCCGCTCGCTGCAGTTCTTCTTCGCCGCGGGCCAGTACAACGATGTGGATTACATCCTGCTGG
CCGGCGGCACCGCTTCCATTCCGGATCTTGATCGCCTGATCCAGCAGAAGATCGGCACCCAGACACTGGTCGCCAACCCC
TTCGCCGATATGGCCCTGAGCGGCAAGGTCAACGCGGGAGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6AGS6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.775

100

0.559
  comM Acinetobacter nosocomialis M2

55.775

100

0.559
  comM Acinetobacter baylyi ADP1

52.542

100

0.525
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment