Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GPY27_RS00800 Genome accession   NZ_CP046761
Coordinates   154678..155271 (+) Length   197 a.a.
NCBI ID   WP_069486246.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain AM46865     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 149678..160271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY27_RS00785 - 150394..152964 (-) 2571 WP_140095567.1 PBP1A family penicillin-binding protein -
  GPY27_RS00790 pilM 153107..154123 (+) 1017 WP_140095565.1 type IV pilus assembly protein PilM Machinery gene
  GPY27_RS00795 pilN 154107..154685 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  GPY27_RS00800 pilO 154678..155271 (+) 594 WP_069486246.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY27_RS00805 pilP 155261..155776 (+) 516 WP_069486245.1 pilus assembly protein PilP Machinery gene
  GPY27_RS00810 pilQ 155808..157556 (+) 1749 WP_180784188.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY27_RS00815 aroK 157745..158263 (+) 519 WP_005381319.1 shikimate kinase AroK -
  GPY27_RS00820 aroB 158295..159395 (+) 1101 WP_069486244.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23077.61 Da        Isoelectric Point: 4.8159

>NTDB_id=407306 GPY27_RS00800 WP_069486246.1 154678..155271(+) (pilO) [Vibrio parahaemolyticus strain AM46865]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=407306 GPY27_RS00800 WP_069486246.1 154678..155271(+) (pilO) [Vibrio parahaemolyticus strain AM46865]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGATAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

73.298

96.954

0.711

Multiple sequence alignment