Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY27_RS00790 Genome accession   NZ_CP046761
Coordinates   153107..154123 (+) Length   338 a.a.
NCBI ID   WP_140095565.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain AM46865     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 148107..159123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY27_RS00775 - 148531..149259 (-) 729 WP_140095571.1 redoxin family protein -
  GPY27_RS00780 oxyR 149430..150338 (+) 909 WP_140095569.1 DNA-binding transcriptional regulator OxyR -
  GPY27_RS00785 - 150394..152964 (-) 2571 WP_140095567.1 PBP1A family penicillin-binding protein -
  GPY27_RS00790 pilM 153107..154123 (+) 1017 WP_140095565.1 type IV pilus assembly protein PilM Machinery gene
  GPY27_RS00795 pilN 154107..154685 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  GPY27_RS00800 pilO 154678..155271 (+) 594 WP_069486246.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY27_RS00805 pilP 155261..155776 (+) 516 WP_069486245.1 pilus assembly protein PilP Machinery gene
  GPY27_RS00810 pilQ 155808..157556 (+) 1749 WP_180784188.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY27_RS00815 aroK 157745..158263 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37808.37 Da        Isoelectric Point: 6.8323

>NTDB_id=407304 GPY27_RS00790 WP_140095565.1 153107..154123(+) (pilM) [Vibrio parahaemolyticus strain AM46865]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFVCGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGMWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=407304 GPY27_RS00790 WP_140095565.1 153107..154123(+) (pilM) [Vibrio parahaemolyticus strain AM46865]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGTGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATGTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment