Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HZS86_RS01505 Genome accession   NZ_CP058719
Coordinates   304255..305748 (-) Length   497 a.a.
NCBI ID   WP_000249348.1    Uniprot ID   B7LGA7
Organism   Escherichia coli strain M159-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299255..310748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZS86_RS01475 gspK 300049..301026 (-) 978 WP_072643195.1 type II secretion system minor pseudopilin GspK -
  HZS86_RS01480 gspJ 301029..301628 (-) 600 WP_072643194.1 type II secretion system minor pseudopilin GspJ -
  HZS86_RS01485 gspI 301625..301996 (-) 372 WP_000820113.1 type II secretion system minor pseudopilin GspI -
  HZS86_RS01490 gspH 301993..302556 (-) 564 WP_001115154.1 type II secretion system minor pseudopilin GspH -
  HZS86_RS01495 gspG 302560..303015 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HZS86_RS01500 gspF 303032..304255 (-) 1224 WP_001173458.1 type II secretion system inner membrane protein GspF -
  HZS86_RS01505 pilB/pilB1 304255..305748 (-) 1494 WP_000249348.1 type II secretion system ATPase GspE Machinery gene
  HZS86_RS01510 gspD 305748..307808 (-) 2061 WP_000498824.1 type II secretion system secretin GspD -
  HZS86_RS01515 gspC 307838..308797 (-) 960 WP_001549326.1 type II secretion system protein GspC -
  HZS86_RS01520 gspS2 308815..309225 (-) 411 WP_001389459.1 type II secretion system pilot lipoprotein GspS-beta -
  HZS86_RS01525 pilD 309291..310100 (-) 810 WP_001469316.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.36 Da        Isoelectric Point: 6.0390

>NTDB_id=406752 HZS86_RS01505 WP_000249348.1 304255..305748(-) (pilB/pilB1) [Escherichia coli strain M159-1]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=406752 HZS86_RS01505 WP_000249348.1 304255..305748(-) (pilB/pilB1) [Escherichia coli strain M159-1]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362