Detailed information NTG0406753

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HZS86_RS01525
• Genome accession: NZ_CP058719
• Coordinates: 309291..310100 (-)
• Length: 269 a.a.
• NCBI ID: WP_001469316.1
• Uniprot ID: Q8VPD1
• Organism: Escherichia coli strain M159-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 304291..315100

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HZS86_RS01510	gspD	305748..307808 (-)	2061	WP_000498824.1	type II secretion system secretin GspD	-
HZS86_RS01515	gspC	307838..308797 (-)	960	WP_001549326.1	type II secretion system protein GspC	-
HZS86_RS01520	gspS2	308815..309225 (-)	411	WP_001389459.1	type II secretion system pilot lipoprotein GspS-beta	-
HZS86_RS01525	pilD	309291..310100 (-)	810	WP_001469316.1	prepilin peptidase PppA	Machinery gene
HZS86_RS01530	sslE	310298..314857 (-)	4560	WP_001469318.1	lipoprotein metalloprotease SslE	-

Sequence

Protein

• Length: 269 a.a.
• Molecular weight: 29480.17 Da
• Isoelectric Point: 8.3829

>NTDB_id=406753 HZS86_RS01525 WP_001469316.1 309291..310100(-) (pilD) [Escherichia coli strain M159-1]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSVQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATIYLQALF

Nucleotide

• Length: 810 bp

>NTDB_id=406753 HZS86_RS01525 WP_001469316.1 309291..310100(-) (pilD) [Escherichia coli strain M159-1]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGTGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGTTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTGATTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCGC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTATCACTGCCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACAATTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-118)

• Type IV leader peptidase family (Peptidase_A24) (130-239)

3D structure

• AlphaFold DB: Q8VPD1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	46.067	99.257	0.457
pilD	Vibrio campbellii strain DS40M4	42.697	99.257	0.424
pilD	Neisseria gonorrhoeae MS11	39.3	95.539	0.375
pilD	Acinetobacter nosocomialis M2	38.113	98.513	0.375
pilD	Acinetobacter baumannii D1279779	37.736	98.513	0.372