Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GO999_RS02830 Genome accession   NZ_CP046674
Coordinates   617004..618731 (-) Length   575 a.a.
NCBI ID   WP_020832718.1    Uniprot ID   -
Organism   Ralstonia nicotianae strain RS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 612004..623731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO999_RS02800 (GO999_02795) - 612627..613088 (+) 462 WP_211906524.1 NUDIX domain-containing protein -
  GO999_RS02805 (GO999_02800) yacG 613081..613296 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  GO999_RS02810 (GO999_02805) zapD 613307..614065 (-) 759 WP_016723794.1 cell division protein ZapD -
  GO999_RS02815 (GO999_02810) coaE 614183..614797 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  GO999_RS02820 (GO999_02815) - 614810..615691 (-) 882 WP_029240316.1 A24 family peptidase -
  GO999_RS02825 (GO999_02820) pilC 615694..616959 (-) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  GO999_RS02830 (GO999_02825) pilF 617004..618731 (-) 1728 WP_020832718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GO999_RS02835 (GO999_02830) - 618979..620256 (-) 1278 WP_016723792.1 HlyC/CorC family transporter -
  GO999_RS02845 (GO999_02840) ispB 620544..621512 (-) 969 WP_081049965.1 octaprenyl diphosphate synthase -
  GO999_RS02850 (GO999_02845) rplU 621791..622102 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  GO999_RS02855 (GO999_02850) rpmA 622140..622400 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  GO999_RS02860 (GO999_02855) obgE 622577..623677 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63754.56 Da        Isoelectric Point: 7.1546

>NTDB_id=406646 GO999_RS02830 WP_020832718.1 617004..618731(-) (pilF) [Ralstonia nicotianae strain RS]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDVDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=406646 GO999_RS02830 WP_020832718.1 617004..618731(-) (pilF) [Ralstonia nicotianae strain RS]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATGTCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAATATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.747

94.957

0.501
  pilB Acinetobacter baumannii D1279779

49.564

99.652

0.494
  pilB Acinetobacter baylyi ADP1

49.376

97.565

0.482
  pilB Legionella pneumophila strain ERS1305867

46.986

98.087

0.461
  pilB Vibrio cholerae strain A1552

44.643

97.391

0.435
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

94.261

0.424
  pilB Vibrio campbellii strain DS40M4

44.343

95.304

0.423
  pilF Thermus thermophilus HB27

38.256

97.739

0.374

Multiple sequence alignment