Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   HWQ50_RS27225 Genome accession   NZ_CP058331
Coordinates   5843258..5843794 (-) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain ACR22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5838258..5848794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ50_RS27205 (HWQ50_27030) - 5838575..5839723 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  HWQ50_RS27210 (HWQ50_27035) - 5841902..5842144 (+) 243 WP_023109353.1 hypothetical protein -
  HWQ50_RS27220 (HWQ50_27045) tfpZ 5842456..5843193 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  HWQ50_RS27225 pilE 5843258..5843794 (-) 537 WP_025921208.1 pilin Machinery gene
  HWQ50_RS27230 (HWQ50_27050) pilB 5844021..5845724 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWQ50_RS27235 (HWQ50_27055) pilC 5845728..5846945 (+) 1218 WP_023103506.1 type II secretion system F family protein Machinery gene
  HWQ50_RS27240 (HWQ50_27060) pilD 5846948..5847820 (+) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HWQ50_RS27245 (HWQ50_27065) coaE 5847817..5848428 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HWQ50_RS27250 (HWQ50_27070) yacG 5848425..5848625 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=404969 HWQ50_RS27225 WP_025921208.1 5843258..5843794(-) (pilE) [Pseudomonas aeruginosa strain ACR22]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=404969 HWQ50_RS27225 WP_025921208.1 5843258..5843794(-) (pilE) [Pseudomonas aeruginosa strain ACR22]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCTTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGTTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393