Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUU85_RS08845 Genome accession   NZ_CP058302
Coordinates   1819865..1821346 (+) Length   493 a.a.
NCBI ID   WP_176256431.1    Uniprot ID   -
Organism   Escherichia coli strain AH65     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1814865..1826346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUU85_RS08825 (HUU85_08830) gspB 1815034..1815453 (-) 420 WP_000461450.1 putative general secretion pathway protein GspB -
  HUU85_RS08830 (HUU85_08835) gspA 1815455..1816924 (-) 1470 WP_000107576.1 type II secretion system protein GspA -
  HUU85_RS08835 (HUU85_08840) gspC 1817104..1817919 (+) 816 WP_001142708.1 type II secretion system protein GspC -
  HUU85_RS08840 (HUU85_08845) gspD 1817903..1819855 (+) 1953 WP_021547174.1 type II secretion system secretin GspD -
  HUU85_RS08845 (HUU85_08850) pilB 1819865..1821346 (+) 1482 WP_176256431.1 type II secretion system ATPase GspE Machinery gene
  HUU85_RS08850 (HUU85_08855) gspF 1821343..1822539 (+) 1197 WP_001109573.1 type II secretion system inner membrane protein GspF -
  HUU85_RS08855 (HUU85_08860) gspG 1822549..1822986 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  HUU85_RS08860 (HUU85_08865) gspH 1822994..1823503 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  HUU85_RS08865 (HUU85_08870) gspI 1823500..1823877 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HUU85_RS08870 (HUU85_08875) gspJ 1823870..1824457 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HUU85_RS08875 (HUU85_08880) gspK 1824450..1825433 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54591.52 Da        Isoelectric Point: 6.1954

>NTDB_id=404608 HUU85_RS08845 WP_176256431.1 1819865..1821346(+) (pilB) [Escherichia coli strain AH65]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQHLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=404608 HUU85_RS08845 WP_176256431.1 1819865..1821346(+) (pilB) [Escherichia coli strain AH65]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACATCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Acinetobacter baumannii D1279779

45.351

89.452

0.406
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

40.966

96.552

0.396
  pilB Acinetobacter baylyi ADP1

48.082

79.31

0.381
  pilF Thermus thermophilus HB27

45.063

80.122

0.361