Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   HWV68_RS14965 Genome accession   NZ_CP058242
Coordinates   2835156..2836160 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain SP1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2830156..2841160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV68_RS14935 (HWV68_14930) yrbG 2831130..2831786 (-) 657 WP_004398801.1 DUF421 domain-containing protein -
  HWV68_RS14940 (HWV68_14935) - 2831984..2832373 (+) 390 WP_004398667.1 TIGR04086 family membrane protein -
  HWV68_RS14945 (HWV68_14940) yajC 2832430..2832696 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  HWV68_RS14950 (HWV68_14945) tgt 2832733..2833878 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  HWV68_RS14955 (HWV68_14950) queA 2833905..2834933 (-) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  HWV68_RS14960 (HWV68_14955) yrzS 2834963..2835163 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  HWV68_RS14965 (HWV68_14960) ruvB 2835156..2836160 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  HWV68_RS14970 (HWV68_14965) ruvA 2836171..2836776 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  HWV68_RS14975 (HWV68_14970) bofC 2836915..2837427 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  HWV68_RS14980 (HWV68_14975) csbX 2837475..2838782 (-) 1308 WP_003246197.1 MFS transporter -
  HWV68_RS14985 (HWV68_14980) iolG 2838853..2839878 (-) 1026 WP_004398990.1 inositol 2-dehydrogenase -
  HWV68_RS14990 (HWV68_14985) yrzF 2840116..2840763 (+) 648 WP_003246159.1 phosphotransferase -
  HWV68_RS14995 (HWV68_14990) - 2840809..2840931 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=404108 HWV68_RS14965 WP_003229718.1 2835156..2836160(-) (ruvB) [Bacillus subtilis strain SP1]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=404108 HWV68_RS14965 WP_003229718.1 2835156..2836160(-) (ruvB) [Bacillus subtilis strain SP1]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518

Multiple sequence alignment