Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWQ66_RS20265 Genome accession   NZ_CP058223
Coordinates   4269971..4271476 (-) Length   501 a.a.
NCBI ID   WP_115725088.1    Uniprot ID   -
Organism   Escherichia coli strain 550     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264971..4276476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ66_RS20235 (HWQ66_20260) gspK 4265798..4266775 (-) 978 WP_115725082.1 type II secretion system minor pseudopilin GspK -
  HWQ66_RS20240 (HWQ66_20265) gspJ 4266772..4267371 (-) 600 WP_115725083.1 type II secretion system minor pseudopilin GspJ -
  HWQ66_RS20245 (HWQ66_20270) gspI 4267368..4267715 (-) 348 WP_115725084.1 type II secretion system minor pseudopilin GspI -
  HWQ66_RS20250 (HWQ66_20275) gspH 4267730..4268284 (-) 555 WP_115725085.1 type II secretion system minor pseudopilin GspH -
  HWQ66_RS20255 (HWQ66_20280) gspG 4268281..4268715 (-) 435 WP_115725086.1 type II secretion system major pseudopilin GspG -
  HWQ66_RS20260 (HWQ66_20285) gspF 4268746..4269969 (-) 1224 WP_115725087.1 type II secretion system inner membrane protein GspF -
  HWQ66_RS20265 (HWQ66_20290) pilB 4269971..4271476 (-) 1506 WP_115725088.1 type II secretion system ATPase GspE Machinery gene
  HWQ66_RS20270 (HWQ66_20295) etpD 4271476..4273416 (-) 1941 WP_238086663.1 variant type II secretion system secretin EtpD -
  HWQ66_RS20275 (HWQ66_20300) gspC 4273444..4274319 (-) 876 WP_115725089.1 type II secretion system protein GspC -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55782.01 Da        Isoelectric Point: 6.4387

>NTDB_id=403984 HWQ66_RS20265 WP_115725088.1 4269971..4271476(-) (pilB) [Escherichia coli strain 550]
MSRVVQNVSESRPLLPFSFSRTQRILLLREQDGNLVFCMEDTPASALLEVRRVAEGPLNVTTLSAEAFEKQLVSSYQRDS
DEARQMMAEIGNEMDFYTVAGALPDREDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREIL
RLHRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVLRLLDKNSVSLDLAALGMSPQN
QRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSLLNPRDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQ
DPDVVLVGEIRDGETAQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCPRCRQPCQ
VSTELAMDMDIPPETTIWQPAGCQHCSFTGYHGRTGIHELLLIDDRIRTAIYQGEGELGITRLAGSRYLTLRGDGRQKVL
AGETSWEEVVRVTESRLQEEK

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=403984 HWQ66_RS20265 WP_115725088.1 4269971..4271476(-) (pilB) [Escherichia coli strain 550]
ATGAGCAGAGTTGTACAGAATGTCAGTGAATCACGTCCCCTTCTGCCTTTTTCCTTTTCCCGTACGCAGAGAATTTTGTT
GCTGCGCGAACAGGATGGGAATCTAGTGTTCTGCATGGAGGACACGCCTGCCAGTGCCCTGTTGGAGGTTCGCAGGGTGG
CTGAAGGTCCTCTGAATGTCACAACACTGTCGGCGGAAGCGTTTGAAAAACAGCTGGTGAGCAGCTATCAACGTGATTCT
GATGAAGCGCGACAAATGATGGCTGAGATTGGTAATGAAATGGATTTTTATACCGTCGCAGGGGCGTTACCTGACCGTGA
GGACTTACTGGATGCGAATGACGATGCGCCGATTATCCGCCTGATTAACGCAATGCTGACTGAGGCAATTAAAGAGAAAG
CCTCAGATATTCATATTGAAACTTACGAACGTCATTTGCAGGTTCGCTTTCGCGTTGATGGAGTTCTGCGGGAGATCCTC
CGTTTACATCGGAATTTGGCTTCGTTACTGATTTCACGCATTAAAGTCATGGCCCGGTTGGATATTGCCGAAAAACGCGT
TCCACAAGATGGCCGCATGGTACTGCGTATCGGTGGTCGGGCGGTGGATGTGCGTGTTTCAACATTGCCTTCAAACCATG
GTGAACGCATCGTGCTGCGTTTACTAGACAAAAATAGCGTTAGTCTCGATCTTGCTGCACTCGGCATGTCGCCGCAGAAT
CAGCGACACATTGATGCGCTGATCCGCCGTCCTCATGGCATTATTTTGGTGACAGGTCCTACAGGGTCGGGTAAAAGCAC
GACGCTTTATGCCGCGTTAAGCCTGCTGAATCCCCGAGACCGCAACATTATGACGGTCGAGGATCCTGTTGAATATGAAC
TGGACGGTATCAGTCAGACCCAGGTCAACCCGAAGGTGGATATGACCTTTGCCCGGAGTCTGCGCGCTATTTTACGTCAG
GATCCGGACGTGGTGCTTGTGGGGGAGATCCGTGACGGTGAAACAGCCCAGATTGCTGTGCAGGCCTCGCTGACAGGTCA
TTTGGTGCTGTCAACGTTACATACGAATAGTGCAGCAGGCGCGCTTTCGCGTCTGCAGGATATGGGCATCCCTCCCTTTC
TGCTTTCCACCTCATTACTGGCTGTTCTGGCCCAGCGACTGGTTCGCACGTTGTGTCCGCGCTGTCGTCAGCCCTGTCAG
GTCAGTACTGAGTTAGCGATGGACATGGACATTCCCCCTGAAACCACGATCTGGCAGCCTGCCGGGTGTCAGCACTGCAG
TTTCACGGGCTATCACGGGCGCACCGGGATCCACGAGCTGTTGCTGATTGACGATCGCATCCGGACGGCAATCTATCAGG
GGGAGGGGGAGCTGGGTATTACCCGCCTGGCAGGAAGTCGCTATCTGACGCTGCGTGGCGACGGGCGACAAAAGGTGCTA
GCCGGTGAGACCAGTTGGGAGGAGGTGGTTCGCGTTACTGAAAGCAGATTGCAGGAAGAGAAATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(124-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.633

97.804

0.407
  pilF Thermus thermophilus HB27

42.149

96.607

0.407
  pilB Vibrio campbellii strain DS40M4

50.384

78.044

0.393
  pilB/pilB1 Synechocystis sp. PCC 6803

46.265

82.834

0.383
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.242

0.381
  pilB Vibrio cholerae strain A1552

48.849

78.044

0.381
  pilB Acinetobacter baylyi ADP1

47.927

77.046

0.369
  pilB Acinetobacter baumannii D1279779

47.179

77.844

0.367
  pilB Legionella pneumophila strain ERS1305867

47.287

77.246

0.365