Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MDS_RS02915 Genome accession   NC_015410
Coordinates   626128..628242 (+) Length   704 a.a.
NCBI ID   WP_013713729.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina NK-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 621128..633242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDS_RS02895 (MDS_0580) pilM 623290..624354 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  MDS_RS02900 - 624354..624929 (+) 576 WP_041769326.1 PilN domain-containing protein -
  MDS_RS02905 (MDS_0581) pilO 624926..625552 (+) 627 WP_013713727.1 type 4a pilus biogenesis protein PilO -
  MDS_RS02910 (MDS_0582) pilP 625549..626073 (+) 525 WP_085982452.1 type 4a pilus biogenesis lipoprotein PilP -
  MDS_RS02915 (MDS_0583) pilQ 626128..628242 (+) 2115 WP_013713729.1 type IV pilus secretin PilQ Machinery gene
  MDS_RS02920 (MDS_0584) aroK 628248..628766 (+) 519 WP_026042192.1 shikimate kinase AroK -
  MDS_RS02925 (MDS_0585) aroB 628818..629921 (+) 1104 WP_013713731.1 3-dehydroquinate synthase -
  MDS_RS02930 (MDS_0586) - 629932..631551 (+) 1620 WP_013713732.1 AAA family ATPase -

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 76063.34 Da        Isoelectric Point: 4.7689

>NTDB_id=40392 MDS_RS02915 WP_013713729.1 626128..628242(+) (pilQ) [Ectopseudomonas mendocina NK-01]
MNNSLSRLGLSVAAFLLSPALLAASLNDLDVAALPGDRVELKLTFDEPITAPRGYTIEQPARIALDLPGVSSRLGTKNRE
IGMGNARSLTVVEAKDRTRLIVNLTNLAPYTTRVEGNTLFVVVGDGPTSAAPVVAAPVVSAAPAVSAPVSRAVAGVRAVS
DIDFQRGEQGEGNIVISLSDSKISPDVQEQGGKIRIDFPKTQLPESLRVRLDVKDFATPVQFVNASASADRASIVVEPTG
LYDYLVFQTDNKLTVSVKPLTEEEAVHRKADRFTYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVRGNITLRLQN
VPWDQALDLVLKTKGLDQRKVGNVLMVAPADEIAAREQQELAVQKQVAELAPLRKDIIQLNYAKASDIAKLYEEGSSGTL
KDDMRGSVIYDDRTNSLIVTLTQERIDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVRWGGNFWVGDKWNAWGKN
GNLGVEENEDGGQVGRFPGDRQTPVNTPFVDLGVAGTSGIGIGFVTNNTILDMQLSAMEKSGSGEVVSQPKVVTSDKETA
KILKGTEVPYQESSSSGATSTSFKEAALSLEVTPQITPDNRVLMEVKVTKDAPDFSVTSSTGGIPAINKNEVNAKVLVAD
GETIVIGGVYSNTQSKSVDKVPFLGDLPFVGRVFRRDLVQDRKAELLVFLTPRIMNNQAIAVNQ

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=40392 MDS_RS02915 WP_013713729.1 626128..628242(+) (pilQ) [Ectopseudomonas mendocina NK-01]
ATGAATAACTCGCTCTCGCGACTCGGTCTGTCTGTTGCGGCCTTTTTGTTATCGCCAGCCCTGCTTGCAGCCAGCTTGAA
TGACCTGGATGTTGCTGCATTACCGGGTGATCGGGTCGAGCTGAAGCTCACGTTCGATGAGCCCATTACAGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGTATTGCTCTTGATCTGCCGGGCGTGTCCAGTCGTCTGGGCACCAAAAATCGCGAG
ATAGGCATGGGGAATGCCCGCAGTCTGACCGTGGTCGAAGCCAAGGATCGGACTCGCCTGATCGTCAACCTGACGAATCT
AGCGCCCTACACCACTCGGGTTGAAGGCAATACCCTGTTCGTGGTCGTGGGCGATGGGCCAACCTCTGCTGCGCCTGTCG
TCGCCGCTCCGGTCGTAAGCGCCGCTCCTGCCGTTTCGGCGCCTGTGTCGCGCGCTGTGGCGGGTGTCCGCGCAGTGAGC
GACATCGACTTTCAGCGTGGCGAGCAGGGTGAGGGAAATATCGTGATTTCCCTGTCTGACTCTAAGATCAGTCCGGATGT
GCAGGAGCAGGGTGGAAAGATCCGCATCGACTTTCCCAAGACCCAGTTGCCGGAGTCGCTGCGTGTTCGGCTGGATGTGA
AGGATTTCGCGACACCGGTACAGTTCGTCAATGCCAGCGCTTCCGCGGATCGTGCGAGCATCGTCGTCGAGCCAACCGGG
CTCTATGATTATCTGGTATTCCAGACGGACAACAAGCTGACGGTCAGCGTGAAGCCCCTCACCGAAGAGGAAGCGGTACA
TCGCAAGGCGGATCGTTTCACCTACAGCGGTGAGAAGCTTTCCCTCAACTTCCAGGACATCGATGTACGTTCGGTGCTGC
AGCTGATCGCGGATTTCACAGATCTGAACCTGGTCGCCAGCGATACGGTTCGTGGCAATATCACCCTGCGTTTGCAGAAC
GTGCCATGGGATCAGGCGCTGGATTTGGTGCTCAAGACCAAGGGCCTCGATCAGCGCAAGGTTGGTAACGTGCTGATGGT
CGCGCCGGCTGACGAGATTGCCGCCCGCGAGCAGCAGGAACTGGCCGTGCAGAAGCAGGTGGCCGAGCTTGCCCCGTTGC
GCAAGGACATCATCCAGCTGAACTACGCCAAGGCGTCCGACATTGCCAAGCTATACGAAGAAGGCTCCTCGGGTACTCTC
AAGGACGACATGCGTGGCTCGGTGATCTATGACGACCGCACCAATAGCTTGATCGTGACCCTGACCCAAGAGCGTATTGA
TGAGTTGCGTCGTATCGTCACTCAGTTAGATATACCGGTTCGACAGGTGATGATCGAGGCTCGAATCGTTGAGGCAAACG
TCGATTATGATAAGGCTCTTGGGGTTCGTTGGGGGGGCAATTTTTGGGTTGGCGATAAGTGGAACGCTTGGGGTAAGAAC
GGTAACTTAGGCGTAGAGGAAAACGAGGATGGTGGGCAGGTTGGGCGTTTTCCTGGCGACCGTCAGACCCCTGTCAATAC
GCCATTTGTTGATTTGGGTGTTGCAGGAACTTCGGGGATCGGTATTGGTTTTGTGACTAATAACACCATTCTCGATATGC
AGCTCAGTGCGATGGAGAAATCTGGCTCCGGTGAGGTCGTTTCTCAGCCAAAGGTTGTAACCTCTGATAAGGAGACTGCG
AAGATTTTGAAGGGTACCGAGGTCCCTTACCAGGAGTCTAGCTCCAGCGGCGCGACCAGCACGTCTTTTAAGGAGGCTGC
GCTATCTCTTGAGGTCACTCCGCAGATTACGCCTGATAACCGAGTGCTAATGGAGGTAAAAGTTACTAAGGATGCTCCTG
ACTTTTCAGTCACTTCTTCGACTGGTGGTATTCCCGCGATCAACAAAAATGAGGTCAATGCCAAGGTTCTGGTGGCTGAT
GGTGAGACGATCGTCATAGGCGGTGTGTATTCCAATACTCAGTCAAAGTCGGTTGATAAGGTGCCGTTCTTGGGGGATTT
GCCTTTTGTCGGTCGCGTGTTCAGGCGTGATCTAGTACAAGATAGAAAGGCCGAATTGCTTGTCTTCCTTACTCCGCGCA
TCATGAACAACCAAGCCATAGCCGTGAATCAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(537-695)

AMIN domain (AMIN)

(39-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.261

100

0.74
  pilQ Acinetobacter baumannii D1279779

39.732

100

0.42
  pilQ Acinetobacter baumannii strain A118

39.732

100

0.42
  comQ Acinetobacter baylyi ADP1

41.11

99.858

0.411

Multiple sequence alignment