Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MDS_RS02895 Genome accession   NC_015410
Coordinates   623290..624354 (+) Length   354 a.a.
NCBI ID   WP_013713726.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina NK-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 618290..629354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MDS_RS02885 (MDS_0578) - 619322..620590 (+) 1269 WP_013713724.1 malic enzyme-like NAD(P)-binding protein -
  MDS_RS02890 (MDS_0579) - 620697..623108 (-) 2412 WP_013713725.1 penicillin-binding protein 1A -
  MDS_RS02895 (MDS_0580) pilM 623290..624354 (+) 1065 WP_013713726.1 pilus assembly protein PilM Machinery gene
  MDS_RS02900 - 624354..624929 (+) 576 WP_041769326.1 PilN domain-containing protein -
  MDS_RS02905 (MDS_0581) pilO 624926..625552 (+) 627 WP_013713727.1 type 4a pilus biogenesis protein PilO -
  MDS_RS02910 (MDS_0582) pilP 625549..626073 (+) 525 WP_085982452.1 type 4a pilus biogenesis lipoprotein PilP -
  MDS_RS02915 (MDS_0583) pilQ 626128..628242 (+) 2115 WP_013713729.1 type IV pilus secretin PilQ Machinery gene
  MDS_RS02920 (MDS_0584) aroK 628248..628766 (+) 519 WP_026042192.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37843.22 Da        Isoelectric Point: 4.4647

>NTDB_id=40391 MDS_RS02895 WP_013713726.1 623290..624354(+) (pilM) [Ectopseudomonas mendocina NK-01]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=40391 MDS_RS02895 WP_013713726.1 623290..624354(+) (pilM) [Ectopseudomonas mendocina NK-01]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCATACGCAGTCGAGCCGCTTCCGCCAAACGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTACTGGCCAAGGCCAAGAGTGGTGTCAAGACAGCAGCCGTT
GCCGTTGCTGGTTCAGCCGTCATCACCAAGACCATCGAGATGGAAGCCGGGCTTTCTGAAGATGATCTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATTCCCTACCCGCTGGAAGAGGTCGCCATCGACTTCGAAGTGCAGGGGCCCGCGCCGC
GTAACCCCGAGCGAGTCGAAGTGCTGCTGGCTGCATGCCGCAAGGAAAACGTCGAGGTCCGCGAAGCGGCGCTGGCGCTT
GCCGGCTTGACCGCAAAAGTGGTGGACGTCGAGGCGTACGCGCTGGAGCGTGCCTACAGCCTGCTGGAGGCGCAGCTGGG
TGGTGGGCACGACGAACTGACGGTTGCGGTGGTTGATATCGGTGCGACCATGACCACGCTTAGCGTTCTGCACAACGGCC
GCACCATCTATACCCGTGAGCAGCTATTCGGCGGCAAGCAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGTCCGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGTGAAGTATTGCAACCGTTCAAAGAGGC
GGTCGTTCAGCAGGTCTCTCGCTCGCTGCAGTTCTTCTTCGCTGCTGGGCAGTTCAACGATGTTGACTACATCCTCCTGG
CTGGCGGCACTGCGTCCATTCCCGATCTTGATCGGCTGATCCAGCAGAAGATCGGTACGCAGACGCTTGTGGCCAATCCC
TTTGCCGACATGGCGCTCAGCAGCAAGGTCAACGCCGGCGCGTTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449

Multiple sequence alignment