Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HW372_RS02085 Genome accession   NZ_CP058217
Coordinates   445148..446629 (-) Length   493 a.a.
NCBI ID   WP_001219902.1    Uniprot ID   A0A0H2VD07
Organism   Escherichia coli Nissle 1917     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 440148..451629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW372_RS02055 (HW372_02030) gspK 441061..442044 (-) 984 Protein_407 type II secretion system minor pseudopilin GspK -
  HW372_RS02060 (HW372_02035) gspJ 442037..442624 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  HW372_RS02065 (HW372_02040) gspI 442617..442994 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  HW372_RS02070 (HW372_02045) gspH 442991..443500 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  HW372_RS02075 (HW372_02050) gspG 443508..443945 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HW372_RS02080 (HW372_02055) gspF 443955..445151 (-) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  HW372_RS02085 (HW372_02060) pilB 445148..446629 (-) 1482 WP_001219902.1 type II secretion system ATPase GspE Machinery gene
  HW372_RS02090 (HW372_02065) gspD 446639..448591 (-) 1953 WP_001514015.1 type II secretion system secretin GspD -
  HW372_RS02095 (HW372_02070) gspC 448575..449390 (-) 816 WP_001142674.1 type II secretion system protein GspC -
  HW372_RS02100 (HW372_02075) gspA 449570..451039 (+) 1470 WP_000107588.1 type II secretion system protein GspA -
  HW372_RS02105 (HW372_02080) gspB 451041..451463 (+) 423 WP_000461446.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54738.65 Da        Isoelectric Point: 6.2412

>NTDB_id=403656 HW372_RS02085 WP_001219902.1 445148..446629(-) (pilB) [Escherichia coli Nissle 1917]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERTVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQSQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=403656 HW372_RS02085 WP_001219902.1 445148..446629(-) (pilB) [Escherichia coli Nissle 1917]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTGCTGAATGAAGATTCAGCCGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCGCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCACTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCGCAACAATCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGTTGCCCTCATTGCCATCAATCCGGTTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VD07

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.681

84.584

0.412
  pilB Acinetobacter baumannii D1279779

45.909

89.249

0.41
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.416

94.523

0.391
  pilB Acinetobacter baylyi ADP1

48.849

79.31

0.387

Multiple sequence alignment