Detailed information NTG0403012

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: GL183_RS01065
• Genome accession: NZ_CP046355
• Coordinates: 209638..210099 (+)
• Length: 153 a.a.
• NCBI ID: WP_000250539.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain 475
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 204638..215099

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GL183_RS01030 (GL183_01045)	-	205131..206189 (+)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-
GL183_RS01035 (GL183_01050)	-	206340..206705 (+)	366	WP_000286415.1	DUF1033 family protein	-
GL183_RS01040 (GL183_01055)	comGA/cglA/cilD	206780..207721 (+)	942	WP_000249555.1	competence type IV pilus ATPase ComGA	Machinery gene
GL183_RS01045 (GL183_01060)	comGB/cglB	207669..208685 (+)	1017	WP_074017570.1	competence type IV pilus assembly protein ComGB	Machinery gene
GL183_RS01050 (GL183_01065)	comGC/cglC	208687..209013 (+)	327	WP_000738625.1	competence type IV pilus major pilin ComGC	Machinery gene
GL183_RS01055 (GL183_01070)	comGD/cglD	209006..209410 (+)	405	WP_000588030.1	competence type IV pilus minor pilin ComGD	Machinery gene
GL183_RS01060 (GL183_01075)	comGE/cglE	209373..209675 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
GL183_RS01065 (GL183_01080)	comGF/cglF	209638..210099 (+)	462	WP_000250539.1	competence type IV pilus minor pilin ComGF	Machinery gene
GL183_RS01070 (GL183_01085)	comGG/cglG	210077..210523 (+)	447	WP_001811371.1	competence type IV pilus minor pilin ComGG	Machinery gene
GL183_RS01075 (GL183_01090)	-	210626..211213 (+)	588	WP_000679779.1	class I SAM-dependent methyltransferase	-
GL183_RS01080 (GL183_01100)	comYH	211274..212227 (+)	954	WP_000345122.1	class I SAM-dependent methyltransferase	Machinery gene
GL183_RS01085 (GL183_01105)	-	212278..213468 (+)	1191	WP_000167765.1	acetate kinase	-
GL183_RS11615	-	213469..213600 (+)	132	WP_000768904.1	hypothetical protein	-
GL183_RS01090 (GL183_01110)	rnpA	213617..213988 (+)	372	WP_000739247.1	ribonuclease P protein component	-
GL183_RS01095 (GL183_01115)	-	213963..214787 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17851.34 Da
• Isoelectric Point: 6.4832

>NTDB_id=403012 GL183_RS01065 WP_000250539.1 209638..210099(+) (comGF/cglF) [Streptococcus pneumoniae strain 475]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=403012 GL183_RS01065 WP_000250539.1 209638..210099(+) (comGF/cglF) [Streptococcus pneumoniae strain 475]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae D39	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae R6	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae TIGR4	94.118	100	0.941
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915