Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUZ63_RS02060 Genome accession   NZ_CP054965
Coordinates   433830..435311 (-) Length   493 a.a.
NCBI ID   WP_001219890.1    Uniprot ID   -
Organism   Escherichia coli strain STIN_95     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428830..440311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUZ63_RS02030 (HUZ63_01995) gspK 429743..430726 (-) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  HUZ63_RS02035 (HUZ63_02000) gspJ 430719..431306 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  HUZ63_RS02040 (HUZ63_02005) gspI 431299..431676 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  HUZ63_RS02045 (HUZ63_02010) gspH 431673..432182 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  HUZ63_RS02050 (HUZ63_02015) gspG 432190..432627 (-) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -
  HUZ63_RS02055 (HUZ63_02020) gspF 432637..433833 (-) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  HUZ63_RS02060 (HUZ63_02025) pilB 433830..435311 (-) 1482 WP_001219890.1 type II secretion system ATPase GspE Machinery gene
  HUZ63_RS02065 (HUZ63_02030) gspD 435321..437273 (-) 1953 WP_001520228.1 type II secretion system secretin GspD -
  HUZ63_RS02070 (HUZ63_02035) gspC 437257..438072 (-) 816 WP_001142675.1 type II secretion system protein GspC -
  HUZ63_RS02075 (HUZ63_02040) gspA 438252..439721 (+) 1470 WP_000107592.1 type II secretion system protein GspA -
  HUZ63_RS02080 (HUZ63_02045) gspB 439723..440142 (+) 420 WP_000461443.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54690.66 Da        Isoelectric Point: 6.2412

>NTDB_id=402376 HUZ63_RS02060 WP_001219890.1 433830..435311(-) (pilB) [Escherichia coli strain STIN_95]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFAPVSPQQAQMFK
YHQLVVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=402376 HUZ63_RS02060 WP_001219890.1 433830..435311(-) (pilB) [Escherichia coli strain STIN_95]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAGATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTTGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCGCGCCCGTATCCCCTCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGTGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.364

89.249

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.58

97.566

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

46.698

86.004

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilF Thermus thermophilus HB27

45.063

80.122

0.361