Detailed information NTG0401642

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GKO36_RS13630
• Genome accession: NZ_CP046145
• Coordinates: 2762343..2762957 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain PEBA20
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2757343..2767957

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKO36_RS13600 (GKO36_13510)	rpsT	2757646..2757912 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GKO36_RS13605 (GKO36_13515)	holA	2757929..2758970 (-)	1042	Protein_2658	DNA polymerase III subunit delta	-
GKO36_RS13610 (GKO36_13520)	-	2759010..2759161 (-)	152	Protein_2659	hypothetical protein	-
GKO36_RS13615 (GKO36_13525)	-	2759202..2759336 (+)	135	WP_003152870.1	YqzM family protein	-
GKO36_RS13620 (GKO36_13530)	comEC	2759355..2761706 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GKO36_RS13625 (GKO36_13535)	-	2761707..2762276 (-)	570	WP_003152868.1	ComE operon protein 2	-
GKO36_RS13630 (GKO36_13540)	comEA	2762343..2762957 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
GKO36_RS13635 (GKO36_13545)	comER	2763016..2763837 (+)	822	WP_014418420.1	late competence protein ComER	-
GKO36_RS13640 (GKO36_13550)	-	2763906..2764643 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
GKO36_RS13645 (GKO36_13555)	rsfS	2764640..2764996 (-)	357	WP_007408260.1	ribosome silencing factor	-
GKO36_RS13650 (GKO36_13560)	yqeK	2765014..2765574 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GKO36_RS13655 (GKO36_13565)	-	2765564..2766133 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GKO36_RS13660 (GKO36_13570)	yhbY	2766144..2766434 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
GKO36_RS13665 (GKO36_13575)	aroE	2766428..2767270 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=401642 GKO36_RS13630 WP_014418419.1 2762343..2762957(-) (comEA) [Bacillus velezensis strain PEBA20]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=401642 GKO36_RS13630 WP_014418419.1 2762343..2762957(-) (comEA) [Bacillus velezensis strain PEBA20]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363