Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FOC72_RS10645 Genome accession   NZ_CP054570
Coordinates   2181533..2183215 (+) Length   560 a.a.
NCBI ID   WP_002894193.1    Uniprot ID   -
Organism   Streptococcus sanguinis strain FDAARGOS_770     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2176533..2188215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOC72_RS10625 (FOC72_10625) - 2176897..2177478 (+) 582 WP_032914017.1 DNA-3-methyladenine glycosylase -
  FOC72_RS10630 (FOC72_10630) - 2177627..2178793 (-) 1167 WP_002894196.1 MFS transporter -
  FOC72_RS10635 (FOC72_10635) - 2178832..2179353 (-) 522 WP_002894195.1 TetR/AcrR family transcriptional regulator -
  FOC72_RS10640 (FOC72_10640) - 2179523..2180410 (+) 888 WP_002894194.1 cation diffusion facilitator family transporter -
  FOC72_RS10645 (FOC72_10645) pilF 2181533..2183215 (+) 1683 WP_002894193.1 GspE/PulE family protein Machinery gene
  FOC72_RS10650 (FOC72_10650) pilT 2183217..2184281 (+) 1065 WP_002894192.1 type IV pilus twitching motility protein PilT Machinery gene
  FOC72_RS10655 (FOC72_10655) - 2184285..2185511 (+) 1227 WP_002894191.1 type II secretion system F family protein -
  FOC72_RS10660 (FOC72_10660) - 2185681..2186142 (+) 462 WP_032914015.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOC72_RS10665 (FOC72_10665) - 2186284..2186742 (+) 459 WP_002894189.1 type II secretion system protein -
  FOC72_RS10670 (FOC72_10670) - 2186815..2187306 (+) 492 WP_002894187.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOC72_RS10675 (FOC72_10675) - 2187439..2187933 (+) 495 WP_002894185.1 hypothetical protein -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62179.28 Da        Isoelectric Point: 4.9464

>NTDB_id=400217 FOC72_RS10645 WP_002894193.1 2181533..2183215(+) (pilF) [Streptococcus sanguinis strain FDAARGOS_770]
MALIAILVQFNLITAAQKEEILQDMPQSNMQLERYLISKGYVTEEDMLKVMSYYYRVPHVNLSQFVIEKEAVEKVSEKVA
KRHGLIPISFTDGEEGEEPKLVVAMADPSNYIALDDVKIVSKMAVEPYVTFRDDIEKYIDQYYSKGEEAQQAATEIEGFN
VDEEIVEEDLEIKNAPVVRLIDSIISQAIKTRTSDIHIEPFEKVVRVRFRVDGTLVENMQLKANAHSAIATRIKIMSGLD
IAERRIPQDGRIETTIDGKEVDMRVSVLPTVFGEKIVIRILSRNATLLSKEELGFSPTNQKLFEDILKAPEGIILLTGPT
GSGKTTTLYTALRELNDVGKNIITVEDPVEYRLEGVNQVQVNNKAGLTFASGLRSILRQDPDIVLLGEIRDEETASIAVR
AAITGHVVLSTIHTNDTASTVNRLVDMGIKPYLVSTATVGIIAQRLIKRICPKCKTEYTVETNEHAGIGIHKGDTLYRGR
GCNYCSGTGYYGRIAIHEIMAVTREIKSLINDGGTTAQLRAEAKKNGMRDLAEEAIDIAKQGTTTIEEAMKIAFSLEGEV

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=400217 FOC72_RS10645 WP_002894193.1 2181533..2183215(+) (pilF) [Streptococcus sanguinis strain FDAARGOS_770]
ATGGCACTAATCGCTATATTGGTTCAATTCAATCTGATAACGGCAGCTCAAAAAGAAGAAATTCTTCAAGATATGCCACA
ATCAAACATGCAGTTAGAGAGATATCTAATCAGCAAGGGCTATGTGACAGAAGAAGATATGCTGAAAGTCATGAGTTACT
ATTACCGTGTACCTCATGTCAATCTATCACAGTTTGTGATAGAAAAGGAAGCTGTCGAGAAGGTTTCAGAAAAAGTTGCT
AAACGTCATGGATTGATTCCGATCTCTTTCACAGATGGGGAAGAGGGAGAAGAACCCAAGTTAGTAGTTGCGATGGCAGA
CCCAAGCAACTATATCGCCCTAGACGACGTTAAAATCGTTTCTAAGATGGCGGTAGAACCTTATGTAACTTTTCGGGATG
ATATTGAGAAGTATATCGATCAATACTATTCCAAAGGAGAAGAAGCCCAACAGGCAGCGACCGAAATCGAAGGTTTTAAT
GTGGATGAAGAGATCGTCGAAGAAGATCTCGAAATCAAAAACGCTCCGGTTGTGCGTTTGATTGACTCGATTATCAGTCA
GGCAATCAAGACGCGGACCAGCGATATCCATATTGAGCCCTTTGAAAAAGTTGTTCGTGTTCGTTTCCGGGTTGACGGGA
CCTTGGTGGAAAACATGCAGTTGAAAGCCAATGCTCATTCAGCCATTGCGACACGGATTAAGATCATGAGTGGTCTGGAC
ATTGCCGAGCGGCGGATTCCTCAGGATGGACGGATTGAGACAACTATCGACGGCAAAGAAGTCGATATGCGGGTTTCAGT
CTTGCCTACTGTATTCGGTGAAAAAATCGTTATTCGGATTTTGAGCCGGAATGCAACTCTCCTCAGTAAAGAGGAGTTGG
GATTCTCGCCAACCAATCAGAAGCTCTTTGAAGATATTCTTAAGGCGCCAGAAGGCATTATCTTGCTGACTGGTCCTACA
GGAAGCGGAAAGACAACCACTCTCTACACAGCGCTCCGTGAGCTCAATGATGTAGGAAAAAATATCATCACCGTTGAAGA
TCCGGTTGAGTACCGCTTGGAAGGGGTCAACCAAGTTCAGGTAAATAACAAAGCTGGACTGACCTTCGCGAGTGGTTTGA
GAAGTATCCTGCGTCAGGACCCGGACATCGTTCTCTTGGGGGAAATTCGGGATGAAGAGACAGCTAGTATCGCGGTTCGG
GCCGCTATCACTGGTCACGTCGTTCTCTCGACTATCCACACCAATGACACAGCTAGTACAGTCAACCGTTTGGTCGATAT
GGGCATCAAGCCTTATCTGGTCTCAACGGCGACTGTCGGTATTATCGCACAGCGCTTGATTAAGCGCATCTGTCCTAAGT
GTAAGACTGAGTACACTGTAGAAACAAATGAACATGCAGGTATCGGTATTCACAAGGGAGACACCTTGTATCGCGGACGC
GGCTGCAACTACTGTAGCGGTACGGGATACTATGGACGTATAGCTATCCACGAAATCATGGCGGTGACAAGGGAGATTAA
ATCACTAATTAATGATGGTGGAACAACAGCACAGCTTCGAGCAGAAGCTAAGAAAAATGGTATGCGAGACTTGGCGGAGG
AGGCCATTGATATCGCAAAACAGGGGACTACAACGATTGAAGAAGCAATGAAGATTGCCTTTAGTCTAGAAGGGGAAGTT
TAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-148)

Type II/IV secretion system protein (T2SSE)

(184-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

39.711

98.929

0.393
  pilB Vibrio cholerae strain A1552

38.693

100

0.391
  pilB Vibrio parahaemolyticus RIMD 2210633

38.693

100

0.391
  pilB Legionella pneumophila strain ERS1305867

38.324

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.42

99.464

0.382
  pilB Vibrio campbellii strain DS40M4

37.699

100

0.38
  pilF Neisseria gonorrhoeae MS11

37.814

99.643

0.377
  pilB Acinetobacter baylyi ADP1

36.972

100

0.375
  pilB Acinetobacter baumannii D1279779

36.918

99.643

0.368

Multiple sequence alignment