Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GH913_RS21710 Genome accession   NZ_CP046019
Coordinates   5019739..5021475 (-) Length   578 a.a.
NCBI ID   WP_033836748.1    Uniprot ID   A0AA44Z4L9
Organism   Xanthomonas citri pv. malvacearum strain HD-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5014739..5026475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH913_RS21695 (GH913_21695) - 5015773..5017386 (+) 1614 WP_005921369.1 HAMP domain-containing sensor histidine kinase -
  GH913_RS21700 (GH913_21700) pilR 5017653..5019107 (+) 1455 WP_033836745.1 sigma-54 dependent transcriptional regulator Regulator
  GH913_RS21705 (GH913_21705) - 5019294..5019461 (-) 168 WP_033836746.1 hypothetical protein -
  GH913_RS21710 (GH913_21710) pilB 5019739..5021475 (-) 1737 WP_033836748.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GH913_RS21715 (GH913_21715) pilA2 5021517..5021930 (-) 414 WP_005921365.1 pilin Machinery gene
  GH913_RS21720 (GH913_21720) comP 5022027..5022455 (-) 429 WP_005921364.1 pilin Machinery gene
  GH913_RS21725 (GH913_21725) pilC 5022800..5024059 (+) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  GH913_RS21730 (GH913_21730) - 5024066..5024929 (+) 864 WP_003491180.1 A24 family peptidase -
  GH913_RS21735 (GH913_21735) coaE 5024943..5025551 (+) 609 WP_005915836.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62690.83 Da        Isoelectric Point: 5.9527

>NTDB_id=400214 GH913_RS21710 WP_033836748.1 5019739..5021475(-) (pilB) [Xanthomonas citri pv. malvacearum strain HD-1]
MNVAIGSNLVGITGIARRLVQDGALDEASARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFIGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVKKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=400214 GH913_RS21710 WP_033836748.1 5019739..5021475(-) (pilB) [Xanthomonas citri pv. malvacearum strain HD-1]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGCGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTACCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAACTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGCCTTTGATGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTCGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCATAGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTAGTGGTCGAGGCAA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTTGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAAAAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACTGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAAGCGGTCGGTTGCGA
TGAGTGCACCGAAGGTTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCAATCGTGC
TGGAAGGCGGGAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTAACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Legionella pneumophila strain ERS1305867

51.384

100

0.514
  pilB Vibrio cholerae strain A1552

48.616

100

0.486
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.25

96.886

0.448
  pilB Vibrio campbellii strain DS40M4

44.991

98.443

0.443

Multiple sequence alignment