Detailed information NTG0040014

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: SPNINV200_RS01290
• Genome accession: NC_017593
• Coordinates: 231838..232836 (+)
• Length: 332 a.a.
• NCBI ID: WP_001809468.1
• Uniprot ID: Q8CWU7
• Organism: Streptococcus pneumoniae INV200
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 226838..237836

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPNINV200_RS01270 (SPNINV200_02370)	leuS	226978..229479 (+)	2502	WP_000011782.1	leucine--tRNA ligase	-
SPNINV200_RS01275 (SPNINV200_02380)	-	229674..230369 (+)	696	WP_001144913.1	GNAT family N-acetyltransferase	-
SPNINV200_RS01280 (SPNINV200_02390)	-	230381..230797 (+)	417	WP_000628392.1	GNAT family N-acetyltransferase	-
SPNINV200_RS01285	-	231612..231704 (-)	93	WP_000970381.1	type I toxin-antitoxin system Fst family toxin	-
SPNINV200_RS01290 (SPNINV200_02400)	ruvB	231838..232836 (+)	999	WP_001809468.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
SPNINV200_RS01295 (SPNINV200_02410)	-	232817..233389 (+)	573	WP_001098900.1	nucleotidyltransferase family protein	-
SPNINV200_RS01300 (SPNINV200_02420)	-	233620..234378 (+)	759	WP_000466719.1	isoprenyl transferase	-
SPNINV200_RS01305 (SPNINV200_02430)	-	234387..235190 (+)	804	WP_000189857.1	phosphatidate cytidylyltransferase	-
SPNINV200_RS01310 (SPNINV200_02440)	eeP	235212..236471 (+)	1260	WP_000900660.1	RIP metalloprotease RseP	Regulator

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37288.73 Da
• Isoelectric Point: 4.5053

>NTDB_id=40014 SPNINV200_RS01290 WP_001809468.1 231838..232836(+) (ruvB) [Streptococcus pneumoniae INV200]
MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPVIEKAGDLVAILNELEPGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRVRDFAQIMGNGVID
DIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAK
AYEHLGYEYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=40014 SPNINV200_RS01290 WP_001809468.1 231838..232836(+) (ruvB) [Streptococcus pneumoniae INV200]
ATGAGTAGAATTTTAGATAATGAGATAATGGGGGATGAGGAGTTAGTAGAACGCACGCTCCGTCCTCAGTATTTACGTGA
ATATATCGGACAGGATAAGGTCAAGGACCAGCTACAAATCTTTATTGAAGCTGCCAAAATGCGGGATGAAGCGCTGGATC
ATGTGCTCTTATTTGGCCCTCCAGGGCTTGGGAAAACAACCATGGCTTTTGTCATTGCCAATGAACTGGGAGTCAATCTC
AAGCAAACGTCTGGTCCTGTTATCGAAAAAGCGGGTGATTTGGTAGCGATTTTGAATGAGTTAGAGCCTGGAGATGTCCT
CTTTATTGACGAGATCCATCGTTTGCCAATGTCAGTGGAAGAGGTGCTTTATAGTGCTATGGAGGACTTCTACATAGATA
TTATGATTGGGGCTGGTGAGGGTAGTCGTAGTGTTCATTTGGAGTTACCACCTTTTACCTTGATTGGTGCGACGACTCGG
GCTGGTATGCTCTCAAATCCACTACGGGCACGTTTTGGGATTACAGGCCATATGGAGTATTATGCCCATGCTGACTTGAC
AGAAATTGTCGAGCGGACGGCAGATATTTTTGAGATGGAAATCACTCATGAGGCAGCATCTGAGTTGGCCCTACGTAGTC
GTGGGACCCCTCGTATTGCCAATCGTCTCCTCAAGCGCGTGCGCGATTTTGCCCAGATAATGGGGAATGGGGTAATTGAT
GATATTATTACCGATAAGGCTTTGACTATGCTGGATGTTGACCATGAAGGTTTGGACTATGTGGATCAAAAAATCCTTCG
TACCATGATTGAGATGTACAGTGGAGGACCTGTTGGTCTAGGAACTCTTTCTGTGAATATCGCCGAAGAGCGTGAGACAG
TTGAAGACATGTATGAGCCTTACTTGATTCAAAAAGGTTTTATCATGCGGACACGGTCTGGACGGGTGGCGACTGCTAAG
GCATATGAGCACTTAGGTTATGAATACAGTGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q8CWU7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	100	100	1
ruvB	Streptococcus pneumoniae D39	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	99.096	100	0.991
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.429	98.193	0.593
ruvB	Helicobacter pylori 26695	50.774	97.289	0.494
ruvB	Synechocystis sp. PCC 6803	49.84	94.277	0.47