Detailed information NTG0040015

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: LAC30SC_RS09300
• Genome accession: NC_015214
• Coordinates: 1854500..1856296 (+)
• Length: 598 a.a.
• NCBI ID: WP_013642467.1
• Uniprot ID: F0TH10
• Organism: Lactobacillus amylovorus
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1848476..1869340	1854500..1856296	within	0

Gene organization within MGE regions

Location: 1848476..1869340

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LAC30SC_RS09265 (LAC30SC_09490)	-	1848476..1848961 (-)	486	WP_013438636.1	threonine/serine exporter family protein	-
LAC30SC_RS09270 (LAC30SC_09495)	-	1848965..1849735 (-)	771	WP_013438637.1	threonine/serine exporter family protein	-
LAC30SC_RS09275 (LAC30SC_09500)	-	1849746..1851287 (-)	1542	WP_013642465.1	ABC-F family ATP-binding cassette domain-containing protein	-
LAC30SC_RS09280 (LAC30SC_09505)	-	1851296..1851670 (-)	375	WP_013438639.1	hypothetical protein	-
LAC30SC_RS09285 (LAC30SC_09510)	-	1851797..1852375 (+)	579	WP_013438640.1	GNAT family N-acetyltransferase	-
LAC30SC_RS09290 (LAC30SC_09515)	-	1852350..1853039 (-)	690	WP_013438641.1	DUF554 domain-containing protein	-
LAC30SC_RS09295 (LAC30SC_09520)	-	1853106..1854338 (-)	1233	WP_013642466.1	LCP family protein	-
LAC30SC_RS09300 (LAC30SC_09525)	pepF	1854500..1856296 (+)	1797	WP_013642467.1	oligoendopeptidase F	Regulator
LAC30SC_RS09305 (LAC30SC_09530)	-	1856350..1856682 (-)	333	WP_013438644.1	ATP-binding protein	-
LAC30SC_RS09310 (LAC30SC_09535)	-	1856848..1857678 (-)	831	WP_013642468.1	type II CAAX prenyl endopeptidase Rce1 family protein	-
LAC30SC_RS09315 (LAC30SC_09540)	-	1857751..1859265 (-)	1515	WP_013642469.1	L-lactate permease	-
LAC30SC_RS09320 (LAC30SC_09545)	-	1859618..1859983 (+)	366	WP_013438647.1	pyridoxamine 5'-phosphate oxidase family protein	-
LAC30SC_RS09325 (LAC30SC_09550)	-	1860053..1860379 (-)	327	WP_046433828.1	DUF4767 domain-containing protein	-
LAC30SC_RS09330 (LAC30SC_09555)	-	1860499..1862118 (-)	1620	WP_000945966.1	recombinase family protein	-
LAC30SC_RS09335 (LAC30SC_09560)	-	1862060..1862971 (-)	912	WP_013642472.1	ATP-binding protein	-
LAC30SC_RS09340 (LAC30SC_09565)	-	1862968..1863762 (-)	795	WP_001205019.1	replication initiator protein A	-
LAC30SC_RS10915	-	1863835..1864221 (-)	387	WP_000998386.1	cysteine-rich VLP domain-containing protein	-
LAC30SC_RS09350 (LAC30SC_09575)	-	1864211..1865878 (-)	1668	WP_009249723.1	relaxase/mobilization nuclease domain-containing protein	-
LAC30SC_RS09355 (LAC30SC_09585)	-	1865850..1866248 (-)	399	WP_001126628.1	plasmid mobilization protein	-
LAC30SC_RS11725 (LAC30SC_09590)	-	1866895..1867086 (-)	192	WP_000841357.1	hypothetical protein	-
LAC30SC_RS09365 (LAC30SC_09595)	-	1867218..1867415 (-)	198	WP_001909500.1	helix-turn-helix domain-containing protein	-
LAC30SC_RS09370 (LAC30SC_09600)	tet(W)	1867418..1869340 (-)	1923	WP_013642473.1	tetracycline resistance ribosomal protection protein Tet(W)	-

Sequence

Protein

• Length: 598 a.a.
• Molecular weight: 68172.07 Da
• Isoelectric Point: 4.6832

>NTDB_id=40015 LAC30SC_RS09300 WP_013642467.1 1854500..1856296(+) (pepF) [Lactobacillus amylovorus]
MAIPTRNEVPENLKWDLTRIFKTDEDWEAAFDKAKDDVEKLGALKGTLTKSGKDLYEGLTKILAVKRDVENIYVYATMSS
DVDTSNSHYLGYVSRVQTLANQFEAVTSFISPEILSIPSDKLAQFKKDEPRLKNYEHYLETITNKRPHTLPAEEEKIIAD
ASDAMGVSENTFNVLTNSDMEYGYVQDDEGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYAAYGQFQNSLASTLSGVVKK
HNYNAKVHKYDSAREAALSENNVPTKVYDTLIQEVDSHLDLLHRYVALRKKILRLKDLQMWDMYVPLTGKPALSYNFEEA
KEVAKKALAPLGEDYLKHVDYIFNNRVIDVVESKNKVTGAYSGGAYDTDPYELLNWEDNVDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFIHEADAKGEPLTA
DTLDDVYGQLNQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTDADRDAYLGFLKAGSSDY
PTEIMKHAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide

• Length: 1797 bp

>NTDB_id=40015 LAC30SC_RS09300 WP_013642467.1 1854500..1856296(+) (pepF) [Lactobacillus amylovorus]
ATGGCAATTCCAACAAGAAATGAAGTCCCTGAAAATTTGAAATGGGACTTAACCAGAATTTTTAAGACAGATGAAGACTG
GGAAGCAGCTTTTGACAAAGCAAAAGACGATGTTGAAAAGCTGGGAGCATTGAAAGGTACTTTGACTAAGTCTGGTAAAG
ATTTGTACGAAGGCTTAACCAAGATCTTGGCAGTTAAGCGCGATGTCGAAAACATCTACGTTTATGCCACTATGTCTAGT
GACGTTGATACTTCTAACTCACACTATTTAGGCTACGTTAGCCGCGTGCAAACTTTGGCTAACCAATTCGAAGCTGTCAC
TAGTTTTATTAGCCCTGAGATCTTGAGCATCCCTAGCGACAAGTTAGCCCAATTTAAAAAGGACGAACCTCGCTTAAAGA
ACTACGAACATTACCTTGAAACGATCACTAACAAGCGTCCCCACACTTTGCCAGCCGAAGAAGAAAAGATCATCGCTGAC
GCTAGCGACGCAATGGGCGTTTCAGAGAACACCTTCAACGTTTTAACCAACTCCGACATGGAATACGGCTACGTTCAAGA
CGACGAAGGCAATATGGAACAATTGTCTGACGGTTTATACTCACTTTTGATCCAATCACAAAACCGTGACGTCAGAAAAG
GCGCCTTTGACACACTTTACGCCGCTTATGGCCAATTCCAAAACTCACTTGCTTCTACGCTTTCTGGCGTGGTAAAGAAG
CACAACTATAACGCTAAGGTGCACAAGTATGACTCAGCTCGCGAAGCTGCATTGTCTGAAAACAACGTACCTACCAAGGT
TTACGACACTTTAATTCAAGAAGTTGATTCTCACCTTGATTTACTCCACCGTTATGTTGCGCTACGTAAGAAGATTTTAA
GACTCAAAGATTTACAAATGTGGGACATGTATGTGCCACTAACTGGTAAACCTGCTCTTTCCTATAACTTCGAAGAAGCA
AAAGAAGTTGCTAAGAAGGCACTCGCTCCACTTGGTGAAGACTACTTGAAGCACGTCGATTACATTTTTAACAACCGCGT
CATCGACGTTGTGGAATCAAAGAACAAGGTCACTGGTGCCTACTCTGGTGGTGCCTACGATACTGATCCATATGAACTTT
TGAACTGGGAAGACAACGTTGATTCACTTTACACATTAGTGCACGAAACTGGTCACTCAGTTCACTCTTGGTACACTCGT
AACACCCAACCTTACGTTTATGGTGACTACCCAATTTTCGTAGCTGAAATTGCTTCAACCACTAACGAAAACATTTTGAC
TGAATACTTCTTGGACCACATCACTGATCCTAAGACGCGTGCCTTTATCTTGAACTACTACCTTGATTCATTTAAGGGTA
CTTTGTTCCGTCAAACTCAATTTGCCGTATTTGAACAATTTATCCATGAAGCTGACGCTAAGGGTGAACCACTTACTGCC
GACACTTTGGATGACGTTTATGGTCAACTTAACCAACACTACTACGGTGACAGCGTTGAACCCGGCGGCGACATTGCACT
TGAATGGTCACGTATTCCACACTTCTACTACAACTTCTACGTTTACCAATATGCGACTGGTTTTGCGGCTGCGACTGCAC
TTGCTAACAAGGTTGTTCATGGTACTGACGCTGATAGGGATGCATACCTTGGCTTCCTTAAGGCTGGTTCAAGTGATTAT
CCAACTGAAATCATGAAGCATGCTGGCGTTGACATGACTAAGCCTGACTACTTGGAAGATGCCTTCAAGACTTTTGAAAA
GCGTTTGAACGAATTTGAAAGCTTGATTGAAAAGTAA

Domains

Predicted by InterproScan.

• Oligopeptidase F (Peptidase_M3_N) (113-181)

• Peptidase family M3 (Peptidase_M3) (203-583)

3D structure

• AlphaFold DB: F0TH10

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	52.189	99.331	0.518